福建省HIV-1毒株亚型分布分析

Subtype Distribution Analysis of HIV-1 Strain in Fujian Province

目的分析福建省HIV-1毒株亚型毒株的分布特点。方法选取福建医科大学孟超肝胆医院就医的312例HIV-1感染者,采用TaKaLa PrimeScipt^(TM) One Step RT-PCR Ver.2和Prime Ex Taq^(TM) Hot Start Version试剂盒对gag基因区和env基因区进行扩增并测定其序列。结果 312例标本结果显示,131例为CRF07_BC(41.99%),112例为CRF01_AE(35.89%),69例为B亚型(22.12%),性传播是主要感染途径。结论福建省HIV-1毒株亚型分布众多,以CRF07_BC、CRF01_AE流行重组型为主,经性传播为主要感染途径。基于此,需加强对福建省HIV-1感染的监测,有针对性的开展行为干预,尤其是性工作者和同性性传播群体的监控,同时结合福建省HIV-1感染情况开展疫苗研发工作,有效防控区域艾滋病传播。

Objective To analyze the distribution characteristics of HIV-1 subtype in Fujian province.Methods Using TaKaLa PrimeScit^TM One Step RT-PCR Ver.2 and Prime Ex Taq^TM Hot Start Verse reagent kit,312 cases of HIV-1 infected patients were selected from Mengchao Hepatobiliary Hospital of Fujian Medical University to amplify the gag gene region and env gene region and determine its sequence.Results The results of 312 specimens showed that 131 cases were CRF07_BC (41.99%),112 cases were CRF01_AE (35.89%),and 69 cases were subtype B (22.12%).Sexual transmission was the main route of infection.Conclusion There are many subtypes of HIV-1 strains in Fujian Province,mainly CRF07_BC and CRF01_ AE,and sexually transmitted as the main route of infection.Based on this,it is necessary to strengthen the monitoring of HIV-1 infection in Fujian Province,conduct targeted behavioral interventions,especially the monitoring of sex workers and same-sex sexually transmitted groups,and carry out vaccine research and development in conjunction with HIV-1 infection in Fujian Province to effectively prevent and control regional HIV transmission.