蛋白芯片法与胶体金法检测结核抗体对结核病的临床价值

Clinical Value of Protein Chip Method and Colloidal Gold Method for Detecting Tuberculosis Antibody Against Tuberculosis

目的分析胶体金法与蛋白芯片法两种方法检测结核抗体对结核病的临床诊断价值。方法纳入本院2018年1—6月收治临床确诊活动性肺结核病患者68例建立观察组,同期纳入进行健康体检的非结核病受检者80例建立对照组,分别应用胶体金法与蛋白芯片法对研究对象进行结核抗体检测,对比检测结果。结果观察组患者经胶体金法检测的阳性率77.94%,对照组受检者经胶体金法检测的阳性率7.50%,观察组检测阳性率更高(P <0.05);观察组患者经蛋白芯片法LAM、16 KDa与38 KDa反应阳性率为61.76%,对照组蛋白芯片法反应阳性率为6.25%,观察组检测阳性率更高(P <0.05);且观察组患者胶体金法检测阳性率显著高于蛋白芯片法(P <0.05)。结论应用胶体金法对结核抗体进行检测的结核病阳性率与蛋白芯片法对比显著更高,胶体金法检测对早期活动性结核患者的诊断具有一定的辅助应用价值。

Objective To analyze the clinical diagnostic value of tuberculosis antibody for tuberculosis by colloidal gold method and protein chip method.Methods A total of 68 patients with clinically diagnosed active tuberculosis were enrolled in the hospital from January to June 2018.80 patients with non-tuberculosis who were included in the physical examination were enrolled in the control group.The method and protein chip method were used to detect tuberculosis antibodies in the study subjects,and the results were compared.Results The positive rate of colloidal gold detection was 77.94% in the observation group,7.50% in the control group by the colloidal gold method,and the positive rate in the observation group was higher (P < 0.05).The positive rate of LAM,16 KDa and 38 KDa in the chip method was 61.76%,the positive rate in the control group was 6.25%,and the positive rate in the observation group was higher (P < 0.05).The positive rate of colloidal gold detection in the observation group was significantly higher than the protein chip method (P < 0.05).Conclusion The positive rate of tuberculosis detected by colloidal gold method is significantly higher than that of protein chip method.Colloidal gold method has certain auxiliary value for the diagnosis of early active tuberculosis patients.