胃癌组织中FLNa、VEGF和MVD的相关性

Relationship between the expression of FLNa,VEGF and MVD in gastric carcinoma

目的研究胃癌组织中细丝蛋白A (filamin A, FLNa)、血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达水平和微血管密度(microvascular density, MVD)的关系,探讨FLNa、 VEGF和MVD之间的相关性及临床意义。方法采用免疫组织化学法检测51例胃癌组织及正常胃黏膜组织中FLNa、 VEGF和CD34的表达情况,结合临床参数分析三者相关性及临床意义。结果胃癌组织中的FLNa阳性表达率低于正常胃黏膜组织[47.06%(24/51) vs 90.20%(46/51),P=0.000];胃癌组织中的VEGF阳性表达率高于正常胃黏膜组织[92.16%(47/51)vs 31.37%(16/51),P=0.000];胃癌组织中的MVD值高于正常胃黏膜组织(18±3 vs 5±1,P=0.001);胃癌组织中FLNa、 VEGF的表达水平及MVD值与患者癌灶的浸润深度、是否存在淋巴结转移、肝转移有关,而与年龄、性别、癌灶部位、组织类型及Borrmann分型无关; FLNa和VEGF在胃癌组织中的表达水平呈负相关(r=-0.490,P=0.000);胃癌组织中FLNa表达阳性组的MVD值低于FLNa表达阴性组(4±1 vs 17±3,P=0.001),而VEGF表达阳性组的MVD值高于VEGF表达阴性组(16±1 vs 3±1,P=0.000)。结论 FLNa、 VEGF的表达水平和MVD值与胃癌的形成和发展相关, FLNa通过抑制VEGF的表达水平,阻遏了肿瘤新生血管的生成,最终抑制胃癌细胞的局部复发和远处转移。

Objective To investigate the expression level of filamin A (FLNa) and vascular endothelial growth factor (VEGF) in gastric carcinoma tissue before evaluating the correlation between the resultant level and microvascular density(MVD) and discover its clinical significance. Methods The expressions of FLNa, VEGF and CD34 in gastric carcinoma tissue and normal gastric tissues of 51 cases were examined immunohistochemically and the correlation between the expression level of those three proteins and the clinicopathologic features were analyzed too. Results The positive expressions of FLNa in gastric carcinoma tissues were lower than that in normal gastric tissues[47.06%(24/51) vs 90.20%(46/51), P =0.000]. The positive expressions of VEGF in gastric carcinoma tissues were higher than that in normal gastric tissues[92.16%(47/51) vs 31.37%(16/51), P =0.000]. The MVD values in cancer tissues was higher than that in normal tissues(18±3 vs 5±1, P =0.001). Expressions of FLNa, VEGF and MVD value were correlated with the depth of tumor invasion,lymph node metastasis, and liver metastasis, but not with patient s age, gender, lesion site of cancer, tissue types and Borrmann typing. Expressions of FLNa were negatively related with expression of VEGF in cancer tissues( r=-0.490,P =0.000). The mean MVD in group with FLNa positive was lower than in that with FLNa negative ( 4± 1 vs 17± 3 , P =0.001), while the mean MVD in group with VEGF positive was higher than in that with VEGF negative( 16±1 vs 3±1, P =0.000). Conclusion During the occurrence and development of gastric carcinoma, there is a close relationship between the expression level of FLNa, VEGF and MVD. The mechanism may be that FLNa inhibits the formation of tumor angiogenesis by cutting down the expression level of VEGF, and therefore inhibits local recurrence and distant metastasis of gastric cancer cells.