摘要
以壳寡糖为原料,采用一锅法合成壳寡糖氨基硫脲,然后再与吡啶甲醛经缩合反应合成壳寡糖氨基硫脲席夫碱,最后与铜离子发生配位反应合成目标化合物壳寡糖氨基硫脲席夫碱铜。采用红外光谱仪(FTIR)、紫外可见光谱仪(UV-Vis)、核磁共振波谱仪(NMR)、电感耦合等离子体质谱仪(ICP-MS)和热重-差热分析(TG-DTA)对壳寡糖衍生物进行结构表征。同时采用体外菌丝生长速率法测定壳寡糖及其衍生物对辣椒疫霉菌、烟草黑胫菌、禾谷镰刀菌的体外抗真菌活性。结果表明,壳寡糖氨基硫脲席夫碱铜质量浓度为1. 0 g/L时,对辣椒疫霉、烟草黑胫、禾谷镰刀菌的抑菌率分别为74. 19%、56. 60%和66. 60%,均优于壳寡糖。
Chitosan oligosaccharide thiosemicarbazide was prepared in one-pot.Then chitosan oligosaccharide thiosemicarbazone was synthesized by the reaction of chitosan thiosemicarbazide with 2-pyridinecarboxaldehyde.Chitosan oligosaccharide thiosemicarbazone Cu(II)complex was thus obtained by the reaction of chitosan thiosemicarbazide with Cu(II)salt.The structure of the synthesized compounds was characterized by infrared(IR)spectroscopy,ultraviolet-visible(UV-Vis)spectroscopy,nuclear magnetic resonance(NMR),inductively coupled plasma(ICP),and thermogravimetry-differential thermal analysis(TG-DTA).Theantifungal behaviors of chitosan oligosaccharide and its derivatives against three crop-threatening pathogenicfungi:Phytophthora capsici(P.capsici),Phytophthora nicotianae(P.nicotianae),Fusarium graminearum(F.graminearum)were investigated by mycelial growth rate method in vitro.The results show that theinhibitory index of chitosan thiosemicarbazone Cu(II)complex against P.capsici,P.nicotianae,F.graminearum is 74.19%,56.60%,66.60%,respectively,and is higher than those observed withchitosan.
作者
暴金平
樊素芳
杨国玉
王志敏
王莹
刘小花
徐翠莲
BAO Jinping;FAN Sufang;YANG Guoyu;WANG Zhimin;WANG Ying;LIU Xiaohua;XU Cuilian(College of Sciences,Henan Agricultural University,Zhengzhou 450002,China)
出处
《应用化学》
CAS
CSCD
北大核心
2019年第5期500-508,共9页
Chinese Journal of Applied Chemistry
基金
河南省科技攻关计划项目(1521021100700
112101110200)
河南省高等学校青年骨干教师资助计划项目(2014GGJS-083)
国家大学生创新创业训练计划项目(201610466022)~~
关键词
壳寡糖
硫脲
Cu(Ⅱ)配合物
抗真菌活性
chitosan oligosaccharide
thiosemicarbazide
Cu(Ⅱ)complex
antifungal activity