花生芽白藜芦醇的影响因素及分子结构研究

Study on the Influencing Factors and Molecular Structure of Resveratrol from Peanut Buds

研究种子、水质和不同取材部位对白藜芦醇含量的影响,并初步探讨花生芽内白藜芦醇的分子结构类型。采取紫外分光光度法测白藜芦醇含量,首先选择2种花生种子,一是到成熟期收获的种子,一是成熟期后延期半月收获的种子,对比二者萌发后白藜芦醇的含量;花生芽培养期间,使用纯净水和配制的营养水,对比花生芽内白藜芦醇含量的变化;生长初期,取1,2,3,4,5,6,7,8 cm长的胚轴及子叶测其含量;酶解花生芽2,3,4,5 cm胚轴,检测酶解后白藜芦醇含量。结果表明,酶解后的白藜芦醇比酶解前含量增加;胚轴在3 cm时含量最高,子叶在胚轴1 cm时含量最高;水质对白藜芦醇无明显影响,而延长收获期,对白藜芦醇含量有明显影响。白藜芦醇有糖化形式存在,花生芽培养用纯净水,使用延期收获的种子,选取3cm左右到胚轴或整株,即可得到经济实效的花生芽。

The effects of seeds,water quality and different parts on the content of resveratrol were studied,and the molecular structure types of resveratrol in peanut buds were preliminarily discussed.The resveratrol content was determined by ultraviolet spectrophotometry.First,two kinds of peanut seeds were selected.One was the seeds harvested at maturity stage,the other is the seeds harvested half a month later than maturity stage.The resveratrol content after germination was compared between the two seeds.Comparison of resveratrol content in peanut bud culture with pure water and nutrient water.The contents of 1,2,3,4,5,6,7,8 cm long hypocotyls and cotyledon were measured at the initial stage of growth.The 2,3,4,5 cm hypocotyls of peanut sprouts were enzymolysis,and the resveratrol content after enzymatic hydrolysis was detected.Results showed that the content of resveratrol increased after enzymatic hydrolysis,the hypocotyls had the highest content at 3 cm and the highest content of cotyledons was 1 cm at the hypocotyl.Water quality had no significant effect on resveratrol,but prolonged harvest period had a significant effect on resveratrol content.Resveratrol exists in the form of saccharification.Peanut buds can be cultured with pure water,delayed harvested seeds,selected about 3 cm to the hypocotyl or whole plant,and the economic and effective peanut buds can be obtained.