TCT阴性CINⅢ级患者的HPV感染状况及感染亚型特点分析

Study on the prevalence of HPV infection and human papilloma virus subtypes among patients with CIN Ⅲ and negative TCT

目的探讨妇女子宫颈薄层液基细胞学检测(TCT)结果为阴性的宫颈上皮内瘤变(CIN)Ⅲ级患者人乳头瘤病毒(HPV)的感染状况及感染亚型特点。方法对100例TCT阴性的CINⅢ级患者(研究组)和100例子宫颈浸润性鳞状细胞癌(SCC)患者(对照组)组织中的DNA片段进行扩增,利用导流杂交技术测定HPV亚型,分析HPV亚型的特点。比较两组患者HPV的总感染率和两组HPV阳性患者的临床特征分布情况。结果研究组患者HPV的总感染率明显低于对照组患者,差异有统计学意义(P﹤0.01)。两组HPV阳性患者中均是单一感染所占比例最高,二重感染所占比例次之,三重感染所占比例和四重感染所占比例最低。研究组与对照组的上述各感染所占比例比较,差异均无统计学意义(P﹥0.05)。两组HPV阳性患者在年龄、民族、性生活史、婚姻状况、职业方面,以及HPV16、HPV58、HPV52、HPV33和HPV18高危型的感染情况比较,差异均无统计学意义(P﹥0.05)。结论 TCT阴性的CINⅢ级患者的HPV感染型别以单一感染为主,HPV16单一感染或联合其他亚型的多重感染可能是其主要特点。

Objective To explore the prevalence of human papilloma virus(HPV)infection and subtypes among patients with cervical intraepithelial neoplasia(CIN)Ⅲwho had negative results obtained from thinprep liquid-based cytology test(TCT).Method The DNA fragments of the tissues from 100 cases with CINⅢand negative TCT results(study group)and from 100 squemous cell carcinoma(SCC)patients(control group)were amplified,and HPV subtypes were determined by diversion hybridization,and the characteristics of HPV subtypes were analyzed.The overall infection rate and distribution of clinical characteristics of positive HPV patients between the two groups were compared.Result The overall HPV infection rate in study group was lower compared with that in control group,showing statistically significant difference(P<0.01).Among all the infections,single-genotype infection had the highest infection percentage,followed by dual,triple,and quadruple infections.There were no statistically significant difference between study group and control group regarding the infection percentage above(P>0.05).Besides,the HPV-positive patients were also similar in regard to age,minority,history of sexual life,marital status,occupation,and infection status of high-risk types including HPV16,HPV58,HPV52,HPV33,and HPV18,with no statistically significant differences noted(P>0.05).Conclusion Single genotype HPV infection is most prevalent among patients with CINⅢand negative TCT,and are characterized by single HPV16 infection and multiple infection of other subtypes.