ASPP蛋白家族成员在结直肠癌组织中的表达及其意义

Expression and significance of apoptosis-stimulating protein of p53 family members in colorectal cancer

目的研究p53凋亡刺激蛋白(apoptosis-stimulating protein of p53, ASPP)家族成员在结直肠癌(colorectal cancer, CRC)组织中的表达及其与临床病理特征的关系。方法采用免疫组织化学方法和免疫荧光染色方法检测115例CRC组织中ASPP蛋白家族成员(ASPP1、ASPP2、iASPP)的表达,并应用统计学方法检验ASPP的表达与CRC临床病理特征之间的关系。结果 ASPP1在115例CRC组织中均无表达,ASPP2的表达率为91.3%(105/115),iASPP的表达率为22.6%(26/115)。ASPP2的表达在≥50岁与<50岁组患者间差异具有统计学意义(P<0.01);0+Ⅰ期CRC中的表达与Ⅲ期相比,差异具有统计学意义(P<0.05);浸润深度上,Tis+T1组与T3组相比,差异具有统计学意义(P<0.01);淋巴结转移分组,N0与N2组间差异具有统计学意义(P<0.01)。iASPP在淋巴结转移分组N0组与N1组相比,差异具有统计学意义(P<0.05)。ASPP2和iASPP的表达与CRC的其他临床病理特征均无相关性。结论 ASPP家族成员在CRC组织中的表达存在差异;ASPP2可用于判断CRC患者的预后。

Objective To detect the expressions of the ASPP family members in the colorectal cancer(CRC) and to explore the relationship between ASPP and clinical-pathological characteristics of CRC.Methods The expression of ASPP family members in 115 cases of CRC was detected by immunohistochemical method and dual immunofluorescence staining examination, at the same time, the relationship between the ASPP and clinical-pathological characteristics of CRC was analyzed by statistical software SPSS 17.0.Results There was no expression of ASPP1 in 115 cases of samples, the expression rates of ASPP2 and iASPP were 91.3% and 22.6% in CRC tissues. There was markedly difference in ASPP2 positive expression between age ≥50 years old and <50 years old group(P<0.01). In 0+Ⅰand Ⅲ in TNM stage, Tis+T1 and T3 in infiltrative depth, N0 and N2 in the lymph nodes metastasis group, there was significan difference in expression of ASSP2(P<0.05). There was difference in expression of iASPP(P<0.05) between N0 and N1 in lymph nodes metastasis group. Expressions of ASPP2 and iASPP were not related with other clinical chracteristics of CRC. Conclusion The expressions of ASPP family members are different in CRC. Expression of ASPP2 will help to assess the prognosis of CRC patients.