PTEN/β-catenin/Nanog在鼻咽癌组织干细胞中表达相关性的研究

Research on the Expression Correlation of PTEN, β-catenin and Nanog in Nasopharygeal Cancer Stem Cell

目的 PTEN与头颈部放疗抵抗相关,β-catenin参与调控肿瘤干细胞,Nanog是一种肿瘤干细胞标记物,本研究通过检测PTEN、β-catenin、Nanog在鼻咽癌组织中的表达,探讨其相关性。方法采用免疫组化SP法检测130例鼻咽癌病理组织芯片中PTEN、β-catenin、Nanog蛋白的表达水平。结果 PTEN主要表达于细胞浆,β-catenin和Nanog主要表达于细胞核。130例鼻咽癌组织中有62例(48%)PTEN免疫染色阴性,其中38例(61%)β-catenin和Nanog表达均为阳性。鼻咽癌组织中PTEN阴性表达与β-catenin阳性表达、Nanog阳性表达均具有相关性,β-catenin阳性表达与Nanog阳性表达呈正相关。结论 PTEN/β-catenin/Nanog轴参与鼻咽癌干细胞调控。

Objective PTEN has been suggested to play a role in radiotherapy of head and neck tumors.β-catenin is involved in regulating cancer stem cells. Nanog is one of the cancer stem cells markers. This study aims to explore the correlation of the mentioned three proteins in nasopharyngeal carcinoma(NPC) tissue stem cells by testing the expression of PTEN,β-catenin and Nanog. Methods Immunohistochemistry was used to detect the differential expression of PTEN,β-catenin(cyteblast) and Nanog(cyteblast) on a tumor issue microarray consisting of paraffin-embedded NPC samples derived from 130 patients. Results Negative immunostaining for PTEN was found in 62 of 130 tumors(48%).Thirty-eight(61%) out of the sixty-two specimens with negative PTEN staining displayed both nucleus β-catenin and Nanog immunoreactivity. Negative expression of PTEN in NPC was significantly associated with nuclear expression of β-catenin and Nanog.The nuclear expression of β-catenin was positively correlated with the Nanog. Conclusion The PTEN/β-catenin/Nanog axis is involved in the regulation of cancer stem cells in NPC.