miR-148a-3p靶向调控PTEN基因表达对黑色素瘤细胞生物学特性的影响

MiR-148a-3p Down Regulation Inhibits Cell Proliferation, Migration and Invasion and Promotes Cell Apoptosis by Enhancing PTEN in Melanoma

目的探讨miR-148a-3p靶向PTEN在黑色素瘤中的表达及其对黑色素瘤增殖、迁移及凋亡的影响。方法收集90例黑色素瘤癌组织及距肿瘤边缘5 cm的正常组织标本,记录患者临床病理资料,检测黑色素瘤组织及癌旁组织中miR-148a-3p、PTEN的表达水平;将miR-148a-3p、PTEN的siRNA转染人黑色素瘤SK-MEL-3细胞系,分别采用CCK8法、Transwell法和流式细胞术检测转染后细胞增殖水平、迁移能力、侵袭能力和凋亡情况。结果与癌旁正常组织相比,黑色素瘤组织中miR-148a-3p表达明显上调,PTEN表达明显下调(P<0.05)。miR-148a-3p表达水平与黑色素瘤淋巴结转移及TNM分期有关(P<0.05),PTEN表达与黑色素瘤淋巴结转移、肿瘤分化程度、TNM分期显著相关(P<0.05)。miR-148a-3p siRNA组PTEN基因的表达水平显著上调,细胞生长和迁移能力明显受到抑制,凋亡水平显著增加(P<0.05);miR-148a-3p siRNA+PTEN siRNA组细胞生长和迁移能力明显增强,细胞凋亡受到抑制(P<0.05)。结论 miR-148a-3p能负向调控PTEN的表达,从而促进黑色素瘤细胞的生长和迁移,并抑制其凋亡。

Objective To investigate miR-148 a-3 p in target regulating the expression of PTEN and its effects on cell proliferation,migration and apoptosis in melanoma. Methods The melanoma tissues and adjacent normal tissues 5 cm away from the tumor edge were collected from 90 cases of melanoma patients. Expression of miR-148 a-3 p and PTEN in both melanoma tissue and adjacent normal tissue were determined. Next, after siRNA of miR-148 a-3 p or PTEN was transfected into melanoma cell line SK-MEL-3, the cell proliferation,invasion, migration and apoptosis of SK-MEL-3 were detected using CCK-8 assay, Transwell assay and flow cytometry respectively. Results Initially, it was observed that miR-148 a-3 p was up-regulated while PTEN was down-regulated in melanoma tissues, when compared with adjacent normal tissues(P<0.05). Besides, expression of miR-148 a-3 p was related with melanoma lymph node metastasis and tumor node metastasis(TNM) stage(P<0.05). The PTEN expression was not only related with melanoma lymph node metastasis and TNM stage,but also tumor differentiation(P<0.05). In addition, miR-148 a-3 p siRNA elevated PTEN expression, and thus suppressed cell proliferation, invasion and migration and enhanced cell apoptosis in melanoma(P<0.05). After transfection of miR-148 a-3 p siRNA, PTEN siRNA promoted cell proliferation, invasion and migration and suppressed cell apoptosis in melanoma. Conclusion In conclusion, miR-148 a-3 p expression was negatively correlated with the PTEN expression. It may promote proliferation, invasion and migration, and inhibit apoptosis of SK-MEL-3 cells by down-regulating PTEN in melanoma.