兴安百里香茎芽增殖途径的植株再生

Plant Regeneration of the Stem Bud Proliferation Pathway of Thymus dahuricus

为探索野生兴安百里香组织培养最佳培养条件获得完整再生植株,以五大连池野生兴安百里香为研究对象,研究外植体类型、消毒时间和培养基种类对兴安百里香茎芽增殖与微枝生根的影响。结果表明:带腋芽茎段为兴安百里香组培的最佳外植体,采用质量浓度0. 1%升汞浸泡4 min的方法消毒效果最佳;最适愈伤组织诱导培养基为MS+6-BA1. 0 mg/L+2,4-D0. 5 mg/L+蔗糖30 g/L+琼脂粉6 g/L;最适茎芽增殖培养基为MS+6-BA1. 0 mg/L+2,4-D0. 5 mg/L;最适微枝生根培养基为MS+6-BA 1. 0 mg/L+2,4-D 0. 8 mg/L。此研究结果为通过工厂化育苗技术培育野生兴安百里香优质观赏苗木奠定了基础。

In order to explore the optimal culture conditions for wild Thymus dahuricus tissue culture to obtain a complete regenerated plant,we studied the effects of explant type,disinfection time and medium variety on stem bud proliferation and microbranched rooting of Thymus dahuricus with Wudalianchi wild Thymus dahuricus.The results showed that: the best explants for tissue culture of Thymus dahuricus were axillary bud stem segment.The best disinfection effect was achieved by soaking for 4 min with a mass concentration of 0.1% mercury.The best medium for callus induction was: MS+6-BA1.0 mg/L+2,4-D0.5 mg/L+ saccharose 30 g/L+ powdered agar 6 g/L.The most suitable medium for stem-bud multiplication was: MS+6-BA1.0 mg/L+2,4-D0.5 mg/L.The best medium for rooting of microtwigs was: MS+6-BA 1.0 mg/L+2,4-D 0.8 mg/L.The results laid a foundation for the cultivation of high quality ornamental seedlings of wild Thymus dahuricus by means of industrial seedling raising technology.