基于代谢组学的西伯利亚白刺耐盐机理研究

Metabolomic study on the mechanism of salinity tolerance of NitrariasibiricaPall

[目的]为研究基于代谢组学的西伯利亚白刺在盐胁迫条件下的耐盐机理。[方法]本文使用LECO-Fiehn Rtx5数据库对盐胁迫前后差异代谢物进行定性及聚类分析,采用富集分析和拓扑分析方法找到差异代谢物参与的代谢途径。[结果]共鉴定出108种差异代谢物,包括51种氨基酸类、22种糖类、11种脂肪酸类、8种有机酸类、7种醇类、6种生物碱、2种维生素及1种碱基衍生物,层次聚类分析将这些代谢物主要分成了两大类,共参与46条代谢途径。西伯利亚白刺对盐胁迫的应答主要依靠小分子渗透调节物质的积累以及糖代谢和TCA循环产生的能量以保障代谢活动的正常进行,进而增强其对盐胁迫环境的抵抗能力。[结论]结果将为深入揭示西伯利亚白刺的耐盐机制及适应性研究提供一定的理论依据。

[Objective] The purpose of present study was to explore the mechanism underlying salinity tolerance of Nitraria sibirica Pall with metabolomics method.[Method] Qualitative and cluster analysis of different metabolites before and after salt stress were analyzed with LECO-Fiehn Rtx5 database. The enrichment analysis and topological analysis were conducted in order to find the metabolic pathways of different metabolites.[Results] A total of 108 different metabolites were identified, including 51 amino acids, 22 saccharides, 11 fatty acids, 8 organic acids,7 alcohols, 6 alkaloids, 2 vitamins, and 1 base derivative. Detected metabolites were mainly divided into two main types by hierarchical cluster analysis and participated in 46 metabolic pathways. The salt stress response of Nitraria sibirica Pall mainly relied on the accumulation of small molecule which slowly and osmotically regulated substances and energy produced by glycol metabolism and TCA cycle to guarantee the normal metabolic activities, which then enhanced its resistance to salt stress environment.[Conclusion] The results will provide a theoretical basis for further revealing the salt-tolerance mechanism and adaptability of Nitraria sibirica Pall.