Urantide对动脉粥样硬化大鼠心脏UⅡ和GPCR14基因与蛋白表达的影响

Effect of Urantide on expression of UⅡ and GPCR14 in atherosclerotic heart

目的观察Urantide对动脉粥样硬化(AS)大鼠心脏UⅡ和GPCR14基因与蛋白表达的影响。方法采用腹腔注射维生素D3及高脂饲料喂养复制大鼠AS模型,随机分为正常组、AS组、辛伐他汀组、Urantide组(3、7和14 d组)。应用免疫组织化学、实时荧光定量聚合酶链反应(qRT-PCR)、Western blotting检测大鼠心脏UⅡ、GPCR14基因和蛋白的表达水平。结果与正常组比较,AS组大鼠心脏血管及病变部位UⅡ及GPCR14阳性表达增加(P <0.05),UⅡ及GPCR14基因与蛋白质的表达水平升高(P <0.05)。与AS组比较,Urantide各给药组大鼠心脏血管及病变部位的UⅡ及GPCR14阳性表达减少(P <0.05),UⅡ及GPCR14基因与蛋白质的表达水平降低(P <0.05)。结论 Urantide对AS大鼠的心脏具有保护作用。

Objective To investigate the effect of Urantide on the expression of U Ⅱ and GPCR14 gene and protein in atherosclerotic heart. Methods A rat model of atherosclerosis (AS) was established by high-fat diet plus intraperitoneal injection of VD3. Rats were randomly divided into Normal group, AS group, Simvastatin group and Urantide group. Expression of U Ⅱ and GPCR14 gene and protein in rat heart was measured by immunohistochemistry, qRT-PCR and Western blotting. Results Positive staining of U Ⅱ and GPCR14 in AS heart tissue were increased significantly when compared with normal group (P < 0.05). Quantification of U Ⅱ and GPCR14 by qPCR and WB further confirmed upregulation of protein expression in AS group (P < 0.05). Increased expression of U Ⅱ and GPCR14 in AS group was abolished by treatment of Urantide (P < 0.05). Conclusions Urantide has significant protective effect on heart in AS rats probably through downregulation of U Ⅱ and GPCR14.