pH响应性树枝状聚合物-金纳米粒子复合药物载体的制备

Preparation of Dendrimer-gold Nanoparticle Composites for pH Responsive Drug Delivery

以表面接枝聚乙二醇链的聚酰胺胺树枝状聚合物(PEG-PAMAM)为纳米载体,在其内部空腔包覆金纳米粒子,在金纳米粒子表面连接硫辛酸改性的阿霉素(LA-DOX),从而间接实现了抗癌药物在PEGPAMAM内的高效负载.同时,LA-DOX中的酰腙键提供pH响应性,实现了药物的pH响应性释放.紫外-可见(UV-Vis)光谱表明,包覆金纳米粒子的PEG-PAMAM纳米载体对LA-DOX的负载能力显著增强.体外细胞实验表明,负载LA-DOX的树枝状聚合物-金纳米粒子复合药物载体具有较强的抗肿瘤能力.

Dendrimer-gold nanoparticle composites were prepared based on poly(ethylene glycol) modified dendrimer(PEG-PAMAM). Gold nanoparticles internal provided an efficient platform for lipoic acid functionalized DoxorubicinLA-DOX linking,leading to the high loading efficiency of anticancer drugs DOX. Meanwhile the hydrazine linker in LA-DOX realized pH sensitive release for nanocarrier. Verified by UV-Vis spectra,the loading efficiency of LA-DOX in dendrimer-gold nanoparticle nanocomposite was improved. Cell Experiment indicated excellent cytotoxicity of dendrimer-gold nanoparticle composite against Hela cells.