海洋细菌和舟形底栖硅藻在辣椒素防污涂层上的附着行为与机制

Attachment Performance and Mechanism of Marine Bacteria and Navicula Tenera on Capsaicin Antifouling Coating

针对目前含辣椒素防污涂层抑菌、抑藻试验主要集中在如金黄色葡萄球菌等特定菌种的情况,为了考察含辣椒素防污涂层对海洋微生物的影响,选取大连黄海海域的天然海洋细菌和舟形底栖硅藻作为研究对象,通过对添加辣椒素海水饱和溶液(60 mg/L)的天然新鲜海水和舟形底栖硅藻进行培养,研究了辣椒素对海洋细菌和底栖硅藻生长活性的影响。通过海洋细菌附着试验、海洋细菌的分层取样试验和底栖硅藻的叶绿素a值测定,研究了辣椒素及辣椒素涂层对海洋细菌和舟形底栖硅藻的作用机制。结果表明,海水饱和溶液浓度的辣椒素并不会对海洋细菌和舟形底栖硅藻的生长活性造成影响;含辣椒素防污涂层能够通过辣椒素的驱避作用有效地抑制海洋细菌在涂层表面附着;而辣椒素对舟形底栖硅藻在涂层上的附着无抑制作用。

Currently the antibacterial and algal experiments of capsaicin antifouling coating mainly focus on the specific bacteria species such as staphylococcus aureus. To analyze the effect of capsaicin-containing antifouling coating on marine microorganisms, Navicula tenera and natural marine bacteria in the Yellow Sea area of Dalian were selected as the research subject. Capsaicin was added to natural fresh seawater and Navicula Tenera to observe the effect of capsaicin seawater saturated solution(60 mg/L) on the biological activity of seawater bacteria and Navicula Tenera. The mechanism of action of capsaicin and capsaicin-containing antifouling coating on marine bacteria and Navicula Tenera was studied through marine bacterial attachment experiments, stratified sampling experiments with marine bacteria, and the determination of chlorophyll a values of Navicula Tenera. The results show that capsaicin in seawater saturated solution concentration does not affect the growth activity of marine bacteria and Navicula Tenera. Capsaicin-containing anti-fouling can effectively inhibit the adhesion of marine bacteria on the coating surface by repellent action. However, the addition of capsaicin to the antifouling coating does not effectively inhibit the adherence of Navicula Tenera to the coating.