膀胱癌术后尿路感染对TGF-β1/Smads信号通路的影响

Effect of urinary tract infection after bladder cancer surgery on TGF-β1/Smads signaling pathway

目的:分析膀胱癌术后尿路感染对TGF-β1/Smads信号通路的影响。方法:选取82例膀胱癌术后尿路感染患者作为研究对象,同时选取同期82例胱癌术后未发生尿路感染的患者作为对照组。采用免疫化学发光法检测TNF-a、IL-1β及PGE2水平,用免疫组化法检测TGF-β1、Smad4及Smad7蛋白表达水平。根据感染程度的不同将研究组分为轻度感染、中度感染、重度感染3组,比较各组之间的TGF-β1、Smad4及Smad7蛋白表达水平。结果:研究组患者的血清TNF-a、IL-1β及PGE2水平均显著高于对照组,差异存在统计学意义(P <0. 01)。相比对照组,研究组患者的TGF-β1及Smad7蛋白表达水平更高(P <0. 01),而Smad4蛋白表达水平更低(P <0. 01)。在TGF-β1及Smad7蛋白表达方面,A组患者最高,其次为B组,C组最低,而Smad4蛋白表达水平在各组相反,差异均存在统计学意义(P <0. 05)。TGF-β1及Smad7蛋白表达水平与血清TNF-a、IL-1β及PGE2水平呈正相关(r=0. 256,0. 113,0. 432,0. 689,0. 124,0. 162; P <0. 01),Smad4蛋白表达水平与上述指标呈负相关(r=-0. 135,-0. 282,-0. 156; P <0. 01)。结论:膀胱癌术后尿路感染可能与造成TGF-β1/Smads信号通路异常改变,这可能与患者的血清炎症介质升高相关,可能会对患者的预后造成不良影响。

Objective: To analyze the effect of urinary tract infection after bladder cancer surgery on the signal pathway of TGF-β1/Smads. Methods: 82 patients with urinary tract infection after bladder cancer surgery were selected as study subjects,and 82 patients without urinary tract infection after cyst cancer surgery during the same period were selected as control group. TNF-a,il-1 and PGE2 levels were detected by immunochemiluminescence assay. According to the degree of infection,the research group was divided into mild infection,moderate infection and severe infection. Results: Serum levels of TNF-a,il-1 and PGE2 in the study group were significantly higher than those in the control group,with statistically significant differences( P < 0. 05). Compared with the control group,TGF-β1 and Smad7 protein expression levels in the study group were higher( P < 0. 05),while Smad4 protein expression levels were lower( P < 0. 05). In terms of the expression of TG TGF-β1 and Smad7 protein,group A had the highest expression,followed by group B and group C,while the expression level of Smad4 protein was the opposite in each group,and the difference was statistically significant( P < 0. 05). TGF-β1 and Smad7 protein expression levels and serum TNF-a,IL-1 beta and PGE2 levels were positively correlated( r = 0. 256,0. 113,0. 256,0. 689,0. 124,0. 162,P < 0. 01) and Smad4 protein expression levels and negatively correlated with the above index( r = 0. 135,0. 282,0. 156,P < 0. 01). Conclusion: Urinary tract infection after bladder cancer surgery may result in abnormal changes in the TGF-iso1/Smads signaling pathway,which may be related to elevated serum inflammatory mediators in patients,and may have adverse effects on the prognosis of patients.