长链非编码RNANEAT1在肝癌组织中的表达及与临床预后的关系

Expression of long-chain non-coding RNA NEAT1 in tissues of hepatocellular carcinoma and its relationship with clinical prognosis

目的检测长链非编码RNA NEAT1(lncRNA-NEAT1)在肝癌、癌旁肝组织中的表达情况,分析其在预测肝癌患者预后的临床价值。方法采集原发性肝癌患者癌组织和癌旁肝组织78例,HE染色进行常规病理检查,使用PCR法检测lncRNA-NEAT1的表达情况,分析lncRNA-NEAT1在肝癌组织和相应癌旁组织中的表达水平及其表达程度与临床病理指标相关性,探讨lncRNA-NEAT1表达量与肝癌患者临床预后的关系。结果 lncRNA-NEAT1在肝癌组织中较癌旁肝组织明显高表达(P<0.001)。肝癌组织中lncRNA-NEAT1的表达水平与肝癌组织的Edmondson分级(P<0.001)、肿瘤大小(P=0.025)、肿瘤包膜完整与否(P=0.024)、血AFP(P<0.01)明显相关,而与性别、年龄、有无肝炎病史、以及肿瘤数目无关(P=0.330、0.511、0.723、0.934)。lncRNA-NEAT1高表达组患者的术后生存率明显低于lncRNA-NEAT1低表达组(P=0.007)。lncRNA-NEAT1高表达、肿瘤直径≥5 cm、无完整包膜、高Edmondson分级和血AFP≥20μg/L均是影响肝癌患者术后生存率的独立危险因素。结论在肝癌组织较癌旁肝组织中呈高表达,其与肝癌患者临床预后和生存密切相关,可作为预测原发性肝癌患者临床预后的独立因子及治疗新靶点。

Objective To detect the expression of long-chain non-coding RNA NEAT1 (lncRNA-NEAT1) in liver cancer and adjacent liver tissues, and to analyze its clinical value in predicting the prognosis of patients with liver cancer. Methods The liver cancer tissues and adjacent noncancerous tissues were collected from 78 patients with primary liver cancer. HE staining was performed for routine pathological examination. The expression of lncRNA-NEAT1 was detected by PCR. Expression levels of lncRNA-NEAT1 in liver cancer tissues and corresponding adjacent tissues were determined, and the correlation between expression levels and clinicopathological parameters were analyzed. In addition, the relationship between the expression of lncRNA-NEAT1 and the clinical prognosis of patients with liver cancer were explored. Results The expression levels of lncRNA-NEAT1 in liver cancer tissues were significantly higher than those in adjacent noncancerous tissues ( P <0.001). The expression levels of lncRNA-NEAT1 in liver cancer tissues were significantly related to Edmondson grade of liver cancer tissues, tumor size, tumor capsule integrity, blood AFP ( P <0.05 or P <0.01),however,which were not associated with gender, age, history of hepatitis, and number of tumors ( P >0.05).The postoperative survival rate in patients with high lncRNA-NEAT1 expression was significantly lower than that in patients with low lncRNA-NEAT1 expression ( P <0.01). High expression of lncRNA-NEAT1, tumor diameter≥5cm, no intact capsule, high Edmondson grade and blood AFP≥20μg/L were independent risk factors affecting postoperative survival rate of patients with liver cancer. Conclusion lncRNA-NEAT1 is highly expressed in liver cancer tissues,as compared with in adjacent noncancerous tissues, which is closely related to the clinical prognosis and survival of patients with liver cancer. Therefore, it can be used as an independent factor and a new therapeutic target for predicting the clinical prognosis of patients with primary liver cancer.