南丹牛Y-SNPs与Y-STRs遗传多样性研究

Genetic Diversity of Y-SNPs and Y-STRs in Nandan Cattle

[目的]分析南丹牛的Y染色体遗传多样性及父系起源。[方法]用PCR扩增、测序及生物信息学方法进行分析。[结果]通过对25头南丹牛的Y-SNPs和Y-STRs分析,发现南丹牛仅包含瘤牛Y3单倍型组,细分为Y3-88-156和Y3-90-156两种单倍型,单倍型频率分别为92%和8%,南丹牛的Y染色体单倍型多样度为0.1533±0.0915。[结论]南丹牛是瘤牛父系起源,其遗传基础很稳定。

[Objective]The aim of this paper was to analyze Y chromosome genetic diversity and the paternal origin of Nandan cattle.[Methods] Polymerase chain reaction amplification, sequencing and bioinformatics were used for this study.[Results] Based on the analysis of Y-SNPs and Y-STRs markers of 25 Nandan cattle male samples, the results indicated that the Nandan cattle belonged to only zebu Y3 haplogroup,was divided into two haplotypes: Y3-88-156 and Y3-90-156,and their haplotype frequencies were 92% and 8%, respectively.The Y chromosome haplotypediversity was 0.1533 ± 0.0915 in Nandan cattle.[Conclusion] Nandan cattle belong to the zebu paternal origin with low paternal genetic diversity, indicating a very stable genetic basis.