摘要
目的探讨肝细胞癌组织中的尾加压素Ⅱ受体(UTR)的表达水平与肝癌分期之间的关系。方法选取2010年1月至2013年3月首都医科大学附属北京佑安医院收治的47例行手术切除的肝细胞癌患者的术后病理标本作为回顾性研究对象。免疫组织化学染色及Western blotting两种办法评价肝细胞癌组织UTR水平,依照UICC/AJCC第7版进行肝细胞癌TNM分期。UTR表达水平与肝癌TNM分期关系采用spearman相关分析进行。结果肝细胞癌组织UTR免疫组织化学染色程度与肝癌TNM分期呈正相关(r=0. 610,P <0. 01),肝细胞癌组织的UTR免疫组织化学染色强度及UTR相对蛋白定量水平随着TNM分期增加而增高;肝细胞癌组织的UTR水平与肝癌分化程度也存在正相关关系(r=0. 709,P=0. 000),肝癌分化越差,肝细胞癌组织的UTR免疫组织化学染色度越强。结论肝细胞癌组织UTR高表达与肝癌的高TNM分期和低分化有关,测定肝癌组织中的UTR表达水平将有助于判断肝癌患者的预后。
Objective To explore the relationship of urotensinⅡreceptor(UTR)level and TNM stagement in hepatocellular carcinoma(HCC)patients.Methods Tumor tissues in 47 patients were selected,who received radical treatment due to hepatocellullar carcinoma in Beijing YouAn Hospital,Capital Medical University from January 2010 to March 2013.Immunohistochemical staining and Western blotting were used to evaluate the level of tissue UTR.TNM Staging was performed according to UICC/AJCC seventh edition.Spearman correlation analysis was used to explore the relationship between UTR level and TNM Staging.Results Degree of UTR immunohistochemical staining in hepatocellular carcinoma tissue was positively correlated with TNM staging of liver cancer(r=0.610,P<0.01).Both of intensitie of UTR immunohistochemical staining and UTR relative protein in hepatocellular carcinoma tissue increased with the increase of TNM staging.There was also a positive correlation between the UTR level of hepatocellular carcinoma and the degree of liver cancer differentiation(r=0.709,P=0.000).The poorer differentiation of liver cancer,the stronger the UTR immunohistochemical staining of hepatocellular carcinoma.Conclusion The higher expression of UTR in hepatocellular carcinoma is associated with higher TNM staging and poorer differentiation of hepatocellular carcinoma.Determination of UTR expression in hepatocarcinoma tissues will help to judge the prognosis of patients with liver cancer.
作者
魏红涛
薛晓伟
刘晖
丁惠国
WEI Hong-tao;XUE Xiao-wei;LIU Hui(Department of Emergency,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Pathology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China;Department of Pathology,Beijing Youan Hospital,Beijing 100069,China)
出处
《临床和实验医学杂志》
2019年第10期1055-1058,共4页
Journal of Clinical and Experimental Medicine