水乳化法制备罗丹明B接枝琼脂糖荧光微球

Preparation of Fluorescent Microparticles Based on Rhodamine B Grafted Agarose by Water-in-Water Emulsification Method

本文旨在制备一种新型的荧光琼脂糖微球。首先通过环氧氯丙烷活化琼脂糖,再与罗丹明B进行化学接枝,制备了一种具有荧光特性的新型琼脂糖化合物。通过荧光分光光度计及X衍射仪检测,发现新型琼脂糖化合物的荧光特性与罗丹明B没有差别,都难以形成晶体。随后通过水水乳化技术进一步制备荧光琼脂糖微球,制备的微球同样具有罗丹明B的荧光性能。通过荧光显微镜和动态光散射检测,可以明显观察到微球,直径约为44μm。通过酶标仪分析其荧光接枝稳定性,验证了微球荧光特性可以长期存在,证明了接枝罗丹明B的必要性。这种琼脂糖荧光微球可能在各种荧光检测应用中具有潜在的用途。

This article aims to prepare a new type of fluorescent agarose microparticles. First, agarose was activated by epichlorohydrin and then chemically grafted with rhodamine B to prepare a novel agarose compound with fluorescence characteristics. Fluorescence spectrophotometer and X-ray diffractometry showed that there was no difference between the fluorescence characteristics of the new agarose compound and rhodamine B, and there was almost no difference in the structure. Fluorescent agarose microparticles were further prepared by water-in-water emulsification technology. The prepared microparticles also had the fluorescence properties of rhodamine B. By fluorescence microscopy and dynamic light scattering detection, microparticles were clearly observed with a diameter of about 44 μm. The fluorescence stability of the microparticles was analyzed by microplate reader. The fluorescence characteristics of the microparticles were confirmed to be long-term. The necessity of grafting rhodamine B was proved. Such agarose fluorescent microparticles may have potential uses in various fluorescence detection applications.