隔药饼灸对动脉粥样硬化兔胆固醇逆转运核受体LXRα的调节作用

Regulatory effect of herbal cake-partitioned moxibustion on cholesterol reverse transport nuclear receptor LXRα in rabbits with atherosclerosis

目的:观察隔药饼灸对动脉粥样硬化兔胆固醇逆转运肝X受体a(LXRa)的影响。方法:将36只雄性新西兰兔按随机数字表法随机分为正常组、模型组、隔药饼灸组和辛伐他汀组,每组9只。模型组、隔药饼灸组和辛伐他汀组采用高脂饲养法造模,共12周。验证造模成功后,正常组不予治疗,模型组予以捆绑,隔药饼灸组予以隔药饼灸治疗,辛伐他汀组予以辛伐他汀治疗,共治疗4周。实验结束后取主动脉及肝脏观察其病理形态变化,取血清及肝脏检测脂质水平,以蛋白质印迹法(WB)和实时荧光定量聚合酶链反应(RT-qPCR)法分别检测LXRa蛋白及mRNA表达水平。结果:与正常组比较,模型组主动脉结构紊乱,管壁粗糙增厚,內膜不光滑,血管平滑肌细胞排列紧密无序,符合主动脉粥样硬化兔模型的特点。与模型组比较,隔药饼灸组和辛伐他汀组兔主动脉结构清晰,肝细胞变性程度减轻,血清总胆固醇、低密度脂蛋白明显降低(均PV0.01),高密度脂蛋白升高(PV0.01),肝脏总胆固醇明显降低(PV0.01);隔药饼灸组和辛伐他汀组兔与模型组比赛,LXRa蛋白(PV0.01或PV0.05)及LXRamRNA(PVO.Ol)表达增加。结论:隔药饼灸可改善主动脉粥样硬化兔主动脉及肝脏的病变情况,调节血脂和肝脏脂质水平,提高肝脏胆固醇逆转运核受体LXRa的表达,促进胆固醇逆转运从而发挥抗动脉粥样硬化的作用。

Objective: To observe the effect of herbal cake-partitioned moxibustion on liver X receptor alpha (LXRa) in rabbits with atherosclerosis. Methods: Thirty-six male New Zea la nd rabbits were ran domly divided into a no rmal group, a model group, a herbal cake-partitioned moxibustion group and a simvastatin group according to the random number table method, with 9 rabbits in each group. Rabbits in the model group, the herbal cake-partitioned moxibustion group and the simvastatin group were modeled by high fat feeding method which took 12 weeks. After verification of the successful model, rabbits in the normal group were not treated, in the model group were bun died, in the herbal cake-partitioned moxibustio n group were treated with herbal cake-partitioned moxibustion, and those in the simvastatin group were treated with simvastatin, all for a total of 4 weeks. At the end of the experiment, the aorta and liver were observed for pathological changes;serum and liver were used to detect lipid levels;Western blot (WB) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect LXRa protein and mRNA expression levels, respectively. Results: Compared with the normal group, the structure of aorta was disordered, the wall was rough and thick, the intima was unsmooth, and the vascular smooth muscle cells were arranged closely and disorderly in the model group, which was consistent with the characteristics of the rabbit model of aortic atherosclerosis. Compared with the model group, the aortic structure was clear;the degree of hepatocyte degeneration was reduced, the serum total cholesterol and low-density lipoprotein levels were significantly decreased (all P<0.01), the high-density lipoprotein level was elevated (P<0.01), and the total liver cholesterol was decreased significantly (P<0.01) in the rabbits of the herbal cake-partitioned moxibustion group and the simvastatin group;compared with the model group, the protein (PvO.Ol or P<0.05) and mRNA (P<0.01) expressions of rabbit LXRa in the herbal cake-partiti oned moxibusti on group and the simvastati n group were in creased. Conclusion: Herbal cake-partitioned moxibustion can improve the aortic and hepatic lesions, regulate blood lipid and liver lipid levels, in crease the expressi on of liver cholesterol reverse tran sport nu clear receptor LXRa, promote reverse cholesterol transport in the rabbits with aortic atherosclerosis, therefore produces an antiatherogenic effect.