Effect of electroacupuncture on c-IAP1 mRNA and C-IAP2 mRNA in synovial tissues of rats with adjuvant arthritis

电针对佐剂性关节炎大鼠滑膜组织c-IAP1mRNA和C-IAP2 mRNA的影响

Objective: Td observe the therapeutic effect of electroacupuncture (EA) at Zusanli (ST 36), Guanyuan (CV 4) and Ashi points on adjuvant arthritis rats, and explore the mechanism of EA treatment of rheumatoid arthritis (RA). Methods: Sixty male rats were randomly divided into a normal group, a model group, a methotrexate group and an EA group, with 15 rats in each group. Rats in the normal group and the model group were routinely raised and did not receive treatment;rats in the methotrexate group received methotrexate at a dose of 0.35 mg/(kg bw), twice a week for 3 weeks;rats in the EA group received acupuncture at Zusanli (ST 36), Guanyuan (CV 4) and Ashi points, and the bilateral Zusanli (ST 36) and Ashi points were connected to EA apparatus, once a day for 3 weeks. The general status, the swelling degree of the toe, the arthritis index (Al) score, the pathological morphology of the ankle joint, and the mRNA expressions of cellular inhibitor of apoptosis protein (c-lAP) 1 and C-IAP2 in joint synovial tissue cells of the rats in each group were observed. Results: The swelling degree of the toe, Al score and mRNA expressions of c-IAPl and C-IAP2 in the model group were significantly higher than those in the normal group (all P<0.05). Compared with the model group, the swelling degree of the toe, Al score and mRNA expressions of c-IAPl and C-IAP2 in the methotrexate group and the EA group improved (P<0.01 or P<0.05);the expressions of c-IAPl mRNA and C-IAP2 mRNA in rat synovial tissues in the EA group were significantly higher than those in the methotrexate group (P<0.01). Conclusion: EA alleviates joint swelling in rats with adjuvant arthritis. The mechanism may be related to suppress!ng mRNA expressions of c-IAPl and C-IAP2, thus to induce apoptosis of synoviocytes.

目的:观察电针刺激足三里、关元和阿是穴对佐剂性关节炎大鼠的治疗作用,探讨电针治疗类风湿性关节炎(RA)的作用机理。方法:将60只雄性大鼠随机分为空白组、模型组、甲氨蝶呤组和电针组,每组15只。空白组、模型组不予治疗,常规饲养;甲氨蝶呤组按0.35mg/(kgbw)<行甲氨蝶呤灌胃,每周2次,连续给药3周;电针组针刺足三里、关元及阿是穴,双侧足三里和何是穴接电针,每日1次,连续治疗3周。观察并检测各组大鼠的一般状态、足跖肿胀度、关节炎指数(AI)评分、踝关节病理形态学及关节滑膜组织细胞凋亡抑制蛋白(c-IAP)1mRNA和c-IAP2mRNA的表达情况。结果:模型组大鼠足跖肿胀度、AI评分及c-IAPlmRNA和C-IAP2mRNA表达均较空白组显著升高(均P<0.05)。与模型组比较,甲氨蝶呤组和电针组大鼠足跖肿胀度、AI评分及c-IAPlmRNA和C-IAP2mRNA表达均有改善(P<0.01或P<0.05);电针组大鼠滑膜组织细胞c-IAPlmRNA和C-IAP2mRNA表达较甲氨蝶吟组显著增高(P<0.01)。结论:电针疗法能够减轻佐剂性关节炎大鼠关节肿胀度,其作用机理可能与抑制凋亡抑制蛋白c-IAPlmRNA和C-IAP2mRNA表达,从而诱导滑膜细胞凋亡有关。