天香胶囊对晕动病模型大鼠神经递质及激素水平的影响

Effects of Tianxiang capsule on neurotransmitters and hormone level in rats with motion sickness

目的观察天香胶囊对旋转刺激装置诱发晕动病模型大鼠神经递质和血浆激素水平的影响。方法将60只雄性SD大鼠按随机数字表法分为空白对照组、模型对照组、阳性药对照组及天香胶囊低、中、高剂量组,每组10只。天香胶囊低、中、高剂量组灌胃天香胶囊药液0.91、1.82、3.64 g/kg,阳性药对照组灌胃东莨菪碱1 mg/kg,给药后1 h,除空白对照组外,其余各组大鼠采用旋转刺激装置诱发大鼠晕动病模型。造模结束后立即观察并记录大鼠粪便、排尿、立毛、颤抖等情况,计算大鼠晕反应指数;采用ELISA法检测各组大鼠前庭核和血浆中组胺(histamine, HIS)含量;放射免疫分析法检测各组大鼠血浆皮质醇(cortisol, Cort)、精氨酸加压素(arginine vasopressin, AVP)含量。结果与模型对照组比较,天香胶囊低、中、高剂量组大鼠晕反应指数[(6.56±2.16)、(6.10±1.35)、(4.46±2.50)比(8.90±2.61)]降低(P<0.05或P<0.01);前庭核中HIS含量[(12.70±3.86)μg/L、(11.45±1.57)μg/L、(10.02±1.30)μg/L比(17.50±4.82)μg/L]降低(P<0.05或P<0.01);天香胶囊高剂量组血浆HIS[(4.24±1.75)μg/L比(7.69±3.06)μg/L]、Cort[(286.90±8.72)ng/ml比(329.26±29.04)ng/ml]、AVP[(16.54±2.48)pg/ml比(22.35±3.08)pg/ml]水平降低(P<0.05或P<0.01)。结论天香胶囊可明显降低晕动病模型大鼠的晕反应指数,其作用机制可能与下调前庭核神经递质HIS和血浆HIS、Cort、AVP水平有关。

To investigate the effects of Tianxiang capsule on Neurotransmitters and Hormone Level of rats with motion sickness. Methods Male SD rats were randomly divided into six groups, including blank control group, model control group, positive drug control group, low-dose, mid-dose and high-dose Tianxiang capsule groups with the method of random digital table, and every group had 10 mice. Except the normal group, the rats in the other groups were intragastrically pre-administered for 1 hour, and the low, medium and high doses of Tianxiang capsule were 0.91, 1.82, 3.64 g/kg, the positive drug control group was given scopolamine 1 mg/kg, and then the rat motion sickness model was induced by a rotary stimulation device. After the modeling, the feces, urine, standing hair, trembling were immediately observed and recorded, and the halo response index of the rats was calculated. The blood from the heart puncture was taken and the vestibular nucleus were put on the ice. Then, the content of histamine (HIS) in the vestibular nucleus and plasma was detected by ELISA. The expression of plasma cortisol (Cort) and arginine vasopressin (AVP) were measured by radioimmunoassay. Results Compared with the model control group, the motion sickness index of rats with low, medium and high doses of Tianxiang capsule (6.56 ± 2.16, 6.10 ± 1.35, 4.46 ± 2.50 vs. 8.90 ± 2.61) significantly decreased (P<0.05 or P<0.01). The HIS content in the vestibular nucleus (12.70 ± 3.86 μg/L, 11.45 ± 1.57 μg/L, 10.02 ± 1.30 μg/L vs. 17.50 ± 4.82 μg/L) significantly decreased (P<0.05 or P<0.01). The plasma content of HIS (4.24 ± 1.75 μg/L vs. 7.69 ± 3.06 μg/L), Cort (286.90 ± 8.72 ng/ml vs. 329.26 ± 29.04 ng/ml) and AVP (16.54 ± 2.48 pg/ml vs. 22.35 ± 3.08 pg/ml) in the high doses of Tianxiang capsule significantly decreased (P<0.05 or P<0.01). Conclusions The Tianxiang capsule could effectively reduce the motion sickness index of rats with motion sicknes, which might be related to the down-regulation of HIS in Vestibule Nucleus and HIS, Cort and AVP in plasma.