乌及健胃颗粒质量标准研究

Quality standard analysis and research for Wuji-Jianwei granules

目的建立乌及健胃颗粒的质量标准。方法采用薄层色谱法对乌及健胃颗粒中柴胡、党参、延胡索药材进行鉴别;采用HPLC法对芍药苷进行含量测定。结果薄层色谱法专属性强,分离度高,鉴别项中阴性对照无干扰;测得芍药苷在0.124 8~0.748 8 μg (r=0.999 6)范围内线性关系良好,平均加样回收率为94.42%,RSD为1.46%,平均含量为0.725 3 mg/g。结论所建立的方法准确、合理,可用于乌及健胃颗粒的质量控制。

Objective To establish the quality standard of Wuji-Jianwei granules. Methods The contents of Bupleuri Radix, Codonopsis Radix, Paeoniae Radix Alba, Corydalis Rhizoma in Wuji-Jianwei granules were identified by using the thin layer chromatography (TLC) method. The contents of peoniflorin was determined by the high performance liquid chromatography (HPLC) method. Results The TLC had strong specificity and high separation, negative control without interference. The linear range was 0.124 8-0.748 8 μg for peoniflorin (r=0.999 6). The average recovery was 94.42%(RSD=1.46%) and the content of peoniflorin was 0.725 3 mg/g. Conclusions The method is accurate and reasonable, and can be used for the quality control of Wuji-Jianwei granules.