白藜芦醇对化疗所致大鼠卵巢损伤的保护作用

Protective Effect of Resveratrol on Ovary Injury Induced by Chemotherapy in Rats

评估白藜芦醇(Res)在预防顺铂(CP)对大鼠卵巢损伤的作用。方法:40只雌性Wistar大鼠采用数字表法随机分为4组。在21天的实验期间,A组(对照组)不予以任何处理,B组(CP模型组)给予生理盐水,C组(低Res组)给予白藜芦醇25mg/(kg·d),D组(高Res组)给予白藜芦醇75mg/(kg·d)。在用药的第15天,除对照组,其余组均腹腔给予单次剂量的顺铂(5mg/kg)。顺铂注射后1周,用酶联免疫吸附(ELISA)法比较各组大鼠血清中雌二醇(E2)、卵泡刺激素(FSH)及抗苗勒管激素(AMH)浓度变化;行卵巢切除术,称量各组卵巢的湿质量;石蜡包埋卵巢,切片后苏木精-伊红(HE)染色观察卵泡发育和各级卵泡计数。结果:各组间血清E2、FSH比较差异无统计学意义(P>0.05)。与对照组比较,CP模型组大鼠血清AMH下降(P<0.05);原始、初级、次级及成熟卵泡数量均减少(P<0.05)。白藜芦醇低剂量、高剂量组与CP模型组比较,各级卵泡数目均增多,血清AMH升高(P<0.05)。而白藜芦醇的低剂量和高剂量组比较,两者的AMH及各级卵泡数差异无统计学意义(P>0.05)。结论:白藜芦醇可以通过维持各级卵泡的数量来预防顺铂化疗引起的卵巢损伤。

To evaluate the effect of resveratrol (Res) in preventing cisplatin (CP) induced ovarian damage in rats. Methods Forty female Wistar rats were randomly separated into four groups. No medication was given to group A (control group) over the 21-day study period, saline was administered to group B (CP group), low-dose resveratrol was given to group C (low Res group), high-dose resveratrol was given to group D (high Res group).On the 15th days of medication, all groups except for group A were treated a single dose of CP in the abdominal cavity. Serum levels of estradiol (E 2), follicular stimulating hormone (FSH) and anti-Mullerian (AMH) were tested by ELISA on the 21th day. All rats underwent oophorectomy one week after CP application. Primordial, primary, secondary and mature follicles were counted microscopically. Results No significant difference was observed among all groups in E 2 and FSH of serum. AMH level was statistically significantly lower in group B than in group C and group D ( P <0.05). The number of primary, primary and mature follicles were also significantly lower in group B than in group C and group D ( P <0.05), but there was no statistical difference between group C and group D in AMH level and follicles number( P >0.05). Conclusion Resveratrol can prevent CP induced ovarian damage by maintaining the numbers of primordial and growing follicles.