荧光定量PCR在疟疾实验室诊断中的应用

Application of fluorescence quantitative PCR in laboratory diagnosis of malaria

分别采用镜检、巢式PCR和荧光定量PCR等3种检测方法对收集到的79份疟疾血样进行检测,并对检测结果进行比较分析。结合患者临床症状和3种检测结果, 79份血样最终确诊74份为疟原虫阳性,阳性率为93.7%(74/79),其中镜检阳性检出率为82.3%(65/79),巢式PCR阳性检出率为82.3%(65/79),荧光定量PCR阳性检出率为93.7%(74/79),荧光定量PCR阳性检出率高于其他两种方法(P <0.05)。镜检和巢式PCR的一致率为78.4%(58/74),镜检和荧光定量PCR的一致率为63.5%(47/74),巢式PCR和荧光定量PCR的一致率为83.8%(62/74)。相比镜检和巢式PCR,荧光定量PCR敏感性和检出率更高,且用时更短。

Seventy-nine malaria samples were examined by microscopy,nested PCR and fluorescence quantitative PCR,and the detection rate was analyzed among the three methods.After combining clinical symptoms of the patients and results of the three methods,74 of the 79 samples were confirmed to be malaria infection,with a positive rate of 93.7%(74/79).Specifically,the detection rate of microscopy,nested PCR and fluorescence quantitative PCR were 82.3%(65/79),82.3%(65/79) and 93.7%(74/79),respectively.The concordance rate between microscopy and nested PCR was 78.4%(58/74),and that between microscopy and fluorescence quantitative PCR was 63.5%(47/74),between nested PCR and fluorescence quantitative PCR was 83.8%(62/74).In addition,the detection rate of fluorescence quantitative PCR was significantly higher than that of the nested PCR(P < 0.05).Compared with microscopy and nested PCR,the fluorescence quantitative PCR has higher sensitivity and positive detection rate,and is less time-consuming.