清肝化痰活血方对非酒精性脂肪性肝病大鼠肝细胞脂肪沉积的影响及其机制探讨

Effect of Qinggan Huatan Huoxue Formula on fatty deposition in liver cell of rats with non-alcoholic fatty liver disease and its mechanism

目的探讨清肝化痰活血方对非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)大鼠肝细胞脂肪沉积的影响及其对固醇调节元件结合蛋白-1c(sterol regulatory element binding protein,SREBP-1c)的调控作用。方法将雄性SD大鼠60只分为正常组、模型组、治疗组各20只,除正常组,其余各组均采用高脂饮食复制NAFLD大鼠模型;造模8周后,每组取10只,验证造模成功后,治疗组予清肝化痰活血中药干预,模型组及正常组予生理盐水。观察各组大鼠肝脏脂肪变性程度,检测血清ALT、AST、γ-GT和肝组织中TC、TG、FFA水平。实时定量PCR法检测肝细胞SREBP-1c mRNA表达水平。结果灌胃治疗4周后,正常组大鼠肝组织正常,模型组大鼠均发生不同程度脂肪变性,治疗组大鼠肝脏脂肪变性程度明显轻于模型组。与正常组比较,模型组血清ALT、AST及γ-GT显著上升,差异有统计学意义(P<0.05);与模型组比较,治疗组血清ALT、AST、γ-GT活性显著下降(P<0.05),肝组织中TC、TG、FFA含量显著降低(P<0.01)。与正常组比较,模型组肝细胞SREBP-1c基因表达水平明显升高(P<0.01);与模型组比较,治疗组SREBP-1c基因表达水平明显下调(P<0.01)。结论清肝化痰活血方可能通过调控肝细胞SREBP-1c基因表达,减轻NAFLD大鼠肝细胞脂肪沉积。

Objective To investigate the effect of Qinggan Huatan Huoxue Formula on fatty deposition in liver cell of rats with non-alcoholic fatty liver disease(NAFLD) and regulation of sterol regulatory element binding protein-1 c.Methods 60 SPF-class male SD rats were selected and divided into normal group,model group,treatment group with 20 in each group.Apart from the normal group,NAFLD rat model was replicated with high fat diet in other groups.After 8 weeks of modeling,10 rats were taken from each group randomly.After verifying the success of modeling,the treatment group was treated with Qinggan Huatan Huoxue Formula,and the model group and normal group were given saline.Serum ALT,AST,γ-GT,and TC,TG and FFA in liver tissue were tested to observe fatty degeneration in liver.The expression of SREBP-1 c mRNA was detected by real time quantitative PCR method.Results After 4 weeks of treatment,the liver tissue of normal group was normal.In the model group,fatty degeneration occurred in different degrees.Compared with the normal group,serum ALT,AST,and R-GGT in the model group were increased significantly with significant differences(P<0.05);Compared with the model group,serum ALT,AST,γ-GT activity were decreased significantly in the treatment group(P< 0.05);the levels of TC,TG,and FFA in liver tissue,were significantly lowered in the treatment group than that in the model group(P<0.01);compared with the normal group,the expression of SREBP-1 c gene in hepatocytes in the model group was increased significantly(P<0.01);compared with the model group,the level of expression in the treatment group was significantly reduced(P<0.01).Conclusion Qinggan Huatan Huoxue Formula can improve liver inflammation by controlling liver cell SREBP-1 c to make it recover from fatty deposition in liver cells of rats with NAFLD.