黄酒生麦曲液化力检测方法的建立和应用

Establishment and Application of Liquefying Power Detection Method for Raw Wheat Qu of Chinese Huangjiu

为了确定黄酒生麦曲液化力的检测方法,本文在工业液化酶检测方法和白酒大曲液化力检测方法的基础上研究了黄酒生麦曲液化力检测的反应体系,并对液化力检测的浸提条件进行单因素试验。结果表明,生麦曲液化力检测的反应体系为:淀粉浓度1 g/L,反应时间10 min,显色波长580 nm,碘-碘化钾溶液添加量1 mL,显色后在15 min内完成测定,经过加标实验发现该方法适用于检测。生麦曲液化力检测的浸提条件为:麦曲粉碎度50目,浸提溶液为稀释10倍的原浓度为0.2 mol/L pH4.60磷酸缓冲液,料液比1∶200 g/mL,浸提时间1 h,浸提温度40℃,优化浸提条件后生麦曲液化力的检测结果提高了19.23%。利用此方法检测不同地区和企业的12个生麦曲样品,液化力在1.22～3.17 U/g范围内,相对标准偏差在0.50%～5.80%,表明此方法具有良好的准确性和普适性。

In order to determine the liquefying power detection method for raw wheat Qu of Chinese Huangjiu,the research studied the reaction system of liquefying power for raw wheat Qu on the basis of industrial liquefying enzyme detection method and liquefying power detection method for Daqu of Chinese liquor.Besides,the extraction conditions of raw wheat Qu were studied by single factor experiment.The results showed that the reaction system was starch concentration of 1 g/L,reaction time of 10 min,wavelength of 580 nm,the amount of iodine-potassium iodide solution of 1 mL,and the measurement completed within 15 minutes after color development.The recovery experiment found that the method was suitable for detection.The raw wheat Qu crushing degree of 50 mesh,the extraction conditions were extraction solution of diluted 10 times of 0.2 mol/L pH4.60 phosphate buffer,material to liquid ratio of 1∶200 g/mL,extraction time of 1 h,extraction temperature of 40℃,the measured value of liquefying power of raw wheat Qu increased by 19.23%.The method was applied to the determination of 12 raw wheat Qu samples from different regions and companies.The liquefying power was in the range of 1.22~3.17 U/g,and the relative standard deviation was between 0.50%~5.80%.The applicability and accuracy of this method were good.