烟草‘云烟87’叶片衰老中WRKY转录因子的表达及功能预测

Expression and Functional Prediction of WRKY Transcription Factors in Leaf Senescence of Tobacco 'Yunyan 87'

WRKY基因家族是一类含有WRKY保守结构域的转录因子(transcription factors, TFs),在植物的生长发育、胁迫应答等过程中起着重要的作用。目前,已在多种植物中鉴定了WRKY基因家族,但是研究烟草中与发育相关的WRKY转录因子功能预测并不多。本研究通过对旺长期(P)、成熟期(M)、衰老1期(M1)、衰老2期(M2)的烟草叶片进行转录组测序,共获得122个WRKY转录因子。利用多种生物信息学软件对家族成员进行了去冗余处理后得到26条WRKY转录因子,进行结构域分析、家族分析、系统进化树分析,结果显示,可将26个候选NtWRKY蛋白分为Ⅰ、Ⅱ和Ⅲ3个亚家族。通过表达量分析显示,在3个亚家族中与逆境胁迫功能相关的WRKY转录因子多与叶片衰老存在正相关,可以预测为衰老相关基因。本研究不仅为研究普通烟草WRKY转录因子在调控生长发育过程中的作用提供了相关的科学依据,也为进一步分析烟草WRKY基因提供了有价值的信息和表达研究。

WRKY gene family is a class of transcription factors(TFs) that contain WRKY conserved domains and plays an important role in plant growth, development, stress response and other processes. Currently, the WRKY gene family has been identified in many plants. However, few studies have been conducted to predict the function of WRKY transcription factors related to development in tobacco. In this study, a total of 122 WRKY transcription factors were obtained by transcriptome sequencing of tobacco leaves at fast growth period( P), maturation period(M), senescence phases-1(M1) and senescence phases-2(M2). A total of 26 WRKY transcription factors were obtained from family members after de-redundancy treatment with various bioinformatics software.The domain analysis, family analysis and phylogenetic tree analysis were carried out. The results showed that26 candidate NtWRKY proteins could be classified into Ⅰ,Ⅱ and Ⅲ 3 subfamilies. Expression analysis indicated that most WRKY transcription factors related to stress function in three subfamilies were positively correlated with leaf senescence and could be predicted as senescence-related genes. This study not only could provide a scientific basis for studying the role of WRKY transcription factor in regu lating growth and development of common tobacco, but also could provide valuable information and expression research for further analysis of WRKY gene in tobacco.