PLLA/PVP纤维三维多孔支架的制备及细胞相容性研究

Three-dimensional Porous Scaffold Based on Poly(L-lactide acid) Polyvinylpyrrolid Fibers and Its Cellular Biocompatibility

以四氢呋喃(THF)/水为共溶剂,在无其它添加剂条件下,通过低温淬火、萃取、洗涤和干燥得到PLLA/PVP纤维三维多孔支架.采用扫描电镜探讨THF/H2O比例、聚合物浓度、PLLA/PVP比例和淬火温度等条件对纤维支架形貌影响,通过水接触角、吸水率和体外生物活性等测试考察PVP添加对亲水性和生物活性影响.实验结果表明,纤维是由PLLA结晶所形成.PVP加入影响了PLLA结晶,但提高了纤维支架的亲水性.最佳的实验条件为THF/H2O=90∶10、聚合物浓度9%(wt)、PILA/PVP=90∶10、淬火温度-10℃,得到了直径为270±73nm均一、规整的纤维三维多孔支架,支架的吸水率、孔隙率和比表面积分别为17.29%、96.36%和13.45m2/g,该孔隙率和比表面积为细胞的粘附、迁移、分化、增殖以及养料的传输提供了很好的条件.

Poly ( L-lactide acid)/polyvinylpyrrolid three-dimensional nanofibers porous scaffold was prepared by low temperature quenching, e*xtraction washing and drying from tetrahydrofuran/water co-solvent without the assistanee of other additives. The effects of THF/H2O ratio, concentration. PLLA/PVP ratio and quenching temperature etc. on the morphology of fibrous scaffold were investigated by SEM. The influence of addition of PVP in water absorption and bioactivity were also investigated through water absorption test and in vitro bioactivity test. The results showed that fibrous scaffold was produced by crystallization of PLLA. The addition of PVP impeded the crystallization of PLLA and increased hydrophilia of fibrous scaffold. The optimum test conditions were as followed: THF/H2O- 90 : 10. concentration 9%(wt), PLLA/PVP=90 : 10 and quenching temperature - 10°C , the uniform three-dimensional nanofibers porous scaffold with diameter of 270±73 nm were obtained. The water absorption, porosity and specific surface areas of fibrous scaffold were 17. 29%? 96. 36% and 13. 45m2/g, respectively. High porosity and specific surface areas will offer good condition of cell adhesion, cell differentiation, cell prolifer and nutrients transport.