猪源P[6]型轮状病毒GST-VP8^*-Z84蛋白的表达及受体结合特征

Expression and receptor binding characteristics of porcine P[6] genotype rotavirus GST-VP8^*-Z84 protein

目的通过基因工程技术表达我国猪源P[6]基因型轮状病毒(rotaviruses,RVs)GST-VP8*-Z84蛋白,研究该蛋白与寡糖、唾液受体的结合特征。方法以猪源P[6]型RV Z84为研究对象,利用GST大肠杆菌表达系统及亲和层析、凝胶过滤层析法纯化得到病毒GST-VP8^*-Z84蛋白,通过ELISA唾液结合实验及寡糖结合实验分析该基因型与唾液及寡糖受体的结合特征。结果猪源P[6] GST-VP8^*-Z84蛋白与黏蛋白核心2具有很好的结合。结论P[6] RV的潜在受体可能是黏蛋白核心,为轮状病毒与受体间的作用机制及研发RV疫苗和高效治疗药物提供实验基础与理论依据。

Objective The gene engineering technique was used to express the P[6] genotype rotavirus (rotaviruses, RVs) GST-VP8*-Z84 protein from pigs, and the binding characteristics of the protein to oligosaccharide and salivary receptor were studied. Methods The GST-VP8^*-Z84 protein was purified by GST Escherichia coli expression system and affinity chromatography using porcine P[6]. Enzyme-linked immunosorbent assay (ELISA) saliva binding test and oligosaccharide binding test were used to analyze the binding characteristics of the genotype to receptors. Results Porcine P[6] GST-VP8^*-Z84 protein bound well to mucin core 2. Conclusions The potential receptor of P[6] RV may be the core of mucin, which may provide the experimental basis and theoretical basis for the mechanism of rotavirus and receptor interaction and the development of RV vaccine and highly effective therapeutic drugs.