荧光光谱仪的校准方法研究及不确定度评定

Calibration Method of Fluorescence Spectrometer and Evaluation of Uncertainty

荧光光谱仪是一种常用的分析仪器,适用于食品、卫生、农业、环境等方面的样品分析,能提供被测样品的激发光谱、发射光谱、荧光强度、量子产率、荧光寿命及荧光偏振等一种或多种信息。介绍适用于检测波长范围为200～1 700nm的荧光光谱仪的校准方法,针对波长示值误差和重复性、检出限、测量线性及峰值强度重复性给出了校准实例和不确定度评价方法。其中波长示值误差的扩展不确定度为0. 20 nm(k=2);检出限为8. 09×10^(-11)g/mL,其扩展不确定度为3. 60×10^(-11)g/mL(k=2);测量线性的相对扩展不确定度为3. 1%(k=2);峰值强度重复性的相对扩展不确定度为2. 0%(k=2)。荧光光谱仪的校准过程及结果评价的描述将为相关仪器的校准提供一个良好的范例。

Fluorescence spectrometer is a common analytical instrument,which is suitable for the analysis in food,health, agriculture and environment areas.It can provide one or more information such as excitation spectrum,emission spectrum, fluorescence intensity,quantum yield, fluorescence lifetime,and fluorescence polarization.The calibration methods and uncertainty evaluation methods for fluorescence spectrometer with detection wavelength ranging from 200 nm to 1 700 nm are introduced,which containing wavelength indication error and repeatability,detection limit,measurement linearity and peak intensity repeatability.The expanded uncertainty of wavelength indication error is 0. 20 nm( k = 2);the limit of detection is 8. 09×10^-11 g /mL with an expanded uncertainty to be 3. 60×10^-11 g /mL( k = 2);the relative expanded uncertainty of measurement linearity is 3. 1%( k = 2);the relative expanded uncertainty of peak intensity repeatability is 2. 0%( k = 2).The calibration process and result evaluation of fluorescence spectrometer described will provide a good example for calibration of related instruments.