光片荧光显微镜长时间的稳定成像

Long-Time Stabilization Imaging by Light Sheet Fluorescent Microscope

利用4f(f为焦距)系统和电子耦合器件相机跟踪样品的位置,通过纳米平移台对机械漂移进行了补偿,使样品始终处于光片的束腰位置,从而获得了最优图像。所提方法可以对几纳米的机械偏移进行补偿。使用自主搭建的光片荧光显微镜对直径为几微米的荧光微球进行了长时间成像,验证了所提方法的可行性。

By using a 4f(f is the focal length) optical setup and a charge coupled device camera,the sample position is tracked.The mechanical drift is compensated by the nano-translation table which ensures the sample always at the beam waist of the light sheet and thus an optimal image is obtained.With the proposed method,the mechanical drift of several nanometers can be compensated.By using the home-made light sheet fluorescence microscopy,the long-time imaging of fluorescent microspheres with diameters of several micrometers is acquired,which confirms the feasibility of the proposed method.