4',5,7-三羟基异黄酮对LPS同时诱导的THP-1细胞向HAEC细胞迁移的影响

Effects of 4',5,7-trihydroxyisoflavone on LPS induced migration of monocytes and endothelial cells

目的探讨4',5,7-三羟基异黄酮对脂多糖(LPS)同时诱导的人单核细胞(THP-1)向人主动脉内皮细胞(HAEC)迁移能力的影响。方法用不同浓度4',5,7-三羟基异黄酮预处理两种细胞30 min,然后用LPS同时作用两种细胞4 h,Transwell迁移实验检测THP-1细胞向HAEC细胞的迁移能力;将THP-1细胞加入到HAEC细胞中共培养1 h,用酶联免疫吸附法检测共培养细胞培养液上清中MCP-1蛋白的浓度。结果加入不同浓度4',5,7-三羟基异黄酮预处理两种细胞30 min后,THP-1细胞的迁移数量及共培养细胞上清液中MCP-1含量和细胞MCP-1 mRNA的表达水平与LPS组比较均呈浓度依赖性减少(P<0.01)。结论 LPS可诱导THP-1细胞与HAEC细胞迁移且促进MCP-1表达;4',5,7-三羟基异黄酮预处理后可明显抑制LPS诱导的THP-1细胞向HAEC细胞的迁移,并明显降低THP-1细胞与HAEC细胞MCP-1的表达。

Objective To investigate the effects of 4',5,7-trihydroxyisoflavone on LPS-induced migration of humanmonocytes and human aortic endothelial cells.Methods After a pretreatment with 4',5,7-trihydroxyisoflavone for 30 minutes and a following induction by LPS for 4 hours,The migration ability of THP-1 cells to HAEC cells was detected by Transwell assay.The THP-1 cells and the HAEC cells were co-cultured for 1 hour,The protein expression of MCP-1 in the supernatant of the co-cultured cells and mRNA expression of MCP-1 in the co-cultured cells were detected by ELISA and real-time PCR respectively.Results After pretreatment with 4',5,7-trihydroxyisoflavone for 30 minutes,the migration ability of THP-1 cells to HAEC cells was remarkably reduced compared with LPS group(P<0.01).The expressions of MCP-1 at both mRNA and protein level in co-cultured cells also showed a dose dependent reduction of pretreatment with 4',5,7-trihydroxyisoflavone compared with the LPS group(P<0.01).Conclusion The bacterial LPS can promote themigration of THP-1 and HAEC cell lines and stimulates the expression of MCP-1 at culture condition.The pretreatment with 4',5,7-trihydroxyisoflavone can neutralize effect of LPS on these two cell lines.