复方中药组分对链脲菌素糖尿病大鼠糖耐量的影响

Influence of compound Chinese medicine components on glucose tolerance in streptozotocin-induced diabetic rats

目的观察复方中药组分对糖尿病的防治作用,并探讨其防治机制。方法腹腔注射链脲佐菌素(给药剂量为55 mg/kg体重)诱导大鼠糖尿病模型,造模成功后将其随机分为空白对照组、模型对照组、药物干预组(中药组分干预小剂量组、中药组分干预大剂量组),共40只,每组10只。药物干预组分别用大剂量和小剂量复方中药组分进行药物干预,空白对照组和模型对照组每天按10 ml/kg体重标准以生理盐水灌胃,观察空腹血糖、糖耐量及胰腺的超氧化物歧化酶(SOD)活力水平和丙二醛(MDA)含量。结果模型对照组的血糖水平显著高于空白对照组,差异有统计学意义(P<0.001);药物干预组0.0、0.5、1.0、2.0 h的血糖水平明显低于模型对照组,差异有统计学意义(P<0.05)。模型对照组的糖耐量水平明显低于空白对照组,复方中药组分干预大剂量组一定时间内的糖耐量水平明显高于模型对照组。模型对照组的SOD水平明显低于空白对照组,MDA水平明显高于空白对照组;中药组分干预大剂量组的SOD水平明显高于模型对照组,MDA水平明显低于模型对照组,差异均有统计学意义(P<0.01)。中药组分干预小剂量组的SOD水平高于模型对照组,差异有统计学意义(P<0.05)。中药组分干预小剂量组的MDA水平明显低于模型对照组,差异有统计学意义(P<0.01)。结论复方中药组分对糖尿病有防治作用。

Objective To observe the preventive and therapeutic effects of compound Chinese medicine components on diabetes mellitus (DM), and to explore its prevention and treatment mechanism. Methods The rat model of DM was induced by intraperitoneal injection of streptozotocin administered at a dose of 55 mg/kg. After a successful modeling, a total of 40 rats were randomly assigned into the blank control group, the model control group and the drug intervention group (the high-dose drug intervention group, the low-dose drug intervention group), 10 cases in each group. The drug intervention group was treated with high- and low-dose of compound Chinese medicine components, the blank control group and the model control group were intragastrically administered with normal saline per day according to the weight standard of 10 ml/kg. The levels of fasting blood glucose (FBG), glucose tolerance, superoxide dismutase (SOD) activity and malondialdehyde (MDA) in pancreas were observed. Results The level of blood glucose of the model control group was significantly higher than that of the blank control group (P<0.001). The levels of blood glucose at 0.0, 0.5, 1.0, 2.0 h of the drug intervention group were significantly lower than those of the model control group (P<0.05). The levels of glucose tolerance of the model control group were significantly lower than those of the blank control group, the levels of glucose tolerance for some time of the compound Chinese medicine component intervention group in a large dose administration were significantly higher than those of the model control group. The level of SOD of the model control group was significantly lower than that of the blank control group, and the MDA of the model control group was higher than that of the blank control group, the level of SOD of the high-dose intervention group was significantly higher than that of the model control group, the level of MDA of the high-dose drug intervention group was greatly lower than that of the model control group (P<0.01). The level of SOD of the low-dose drug intervention group was higher than that of the model control group, with statistical difference (P<0.05). The MDA in the low-dose drug intervention group was much lower than that in the model control group, with statistical significance (P<0.01). Conclusion Compound Chinese herbal medicines have preventive and therapeutic effects on diabetes mellitus.