多重耐药鲍曼不动杆菌携带金属酶与整合酶基因特征及同源性分析

Gene Characteristics of Metallo-β-Lactamases and Integrase Genes of Multi-drug Resistant Acinetobacter Baumannii and Homology Analysis

目的了解吉林地区多重耐药鲍曼不动杆菌(Multidrug-resistant Acinetobacter baumanni,MDRAB)金属酶与整合酶基因的类型,并对菌株进行同源性分析.方法收集吉林地区2所三级甲等医院临床标本分离鉴定的鲍曼不动杆菌38株.多重耐药株携带金属酶的初筛实验-双纸片协同试验:金属酶与整合酶基因的检测-聚合酶链反应(PCR),并对其进行同源性分析.结果在所有MDRAB菌株亚胺培南-EDTA协同试验中,15株(39.4%)阳性,16株(42.1%)携带整合酶基因Ⅰ,2株(5.2%)携带整合酶基因Ⅱ,2株(5.2%)携带blaVIM型金属酶基因,1株(2.6%)携带blaNDM-1型金属酶基因,未检测出金属酶基因blaIMP,blaSIM-1和整合酶基因Ⅲ.结论该地区临床分离的MDRAB耐药率高,可能与其携带金属酶基因和整合酶基因有关,A型MDRAB为医院感染主要流行株.

Objective To explore gene types of metallo-β-lactamases and integrase genes of multi-drug resistant Acinetobacter baumannii(MDRAB)in Jilin area,and to analyze the homology of all the MDRAB.Method 38 strains of MDRAB were isolated from two grade iii-a general hospital in Jilin area.Preliminary screening test of multiple resistant strains carrying metal enzyme-carbapenems-EDTA double disks testing;detection of metal enzyme and integrase genepolymerase chain reaction(PCR);MDRAB were analyzed for homology by repetitive extragenic palindromic sequence-based PCR.Results 15 strains(39.4%)was positive by Imipenem-EDTA doubles disks test in all MDRAB strains.16 strains(42.1%)carried the intⅠand 2 strains(5.2%)carried the intⅡ.2 strains(5.2%)carried the bla VIM gene,1 strain(2.6%)carried the bla NDM-1 gene,bla IMP,bla SIM-1 and intⅢgenes were undetected.Conclusion The drug resistance rate of clinical isolated MDRAB in this area is high,it may be related to carriage of metallo-β-lactamasesgenes and integrase genes.Type A MDRAB is the main epidemic strain.