β-隐黄素对大鼠实验性牙周炎炎症因子的影响

Effect of β-cryptoxanthin on Level of Inflammatory Factors in Experimental Periodontitis

目的:研究β-隐黄素对大鼠实验性牙周炎炎症因子的影响并探讨其相关作用机制。方法:30只雄性SD大鼠随机分为正常组(N组)、牙周炎组(P组)、β-隐黄素干预组(E组),每组10只。采取结扎双侧上颌第二磨牙颈部联合注射脂多糖(lipopolysaccharide,LPS)诱导牙周炎模型。E组同时于相同位点注射β-隐黄素(每只12μL),每48 h注射1次,共3次。实验第7天采血并立即分离双侧上颌骨,右侧颌骨行组织学分析,血清及左侧分离的牙龈组织以酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法检测细胞白介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、前列腺素E2(prostaglandin E2,PGE_2)含量。结果:E组和P组大鼠IL-1β、TNF-α、PGE_2水平明显高于N组,且炎症细胞浸润较多,牙槽骨吸收明显。与P组比较,E组中IL-1β、TNF-α、PGE_2水平明显降低(P<0.05),炎症细胞浸润较少,牙槽骨吸收减轻。结论:β-隐黄素可能通过降低组织中的炎症因子水平,减少炎症细胞浸润,从而减轻牙周组织的炎症反应,减少牙槽骨吸收。

Objective:To study the effect ofβ-cryptoxanthin on inflammatory factors in rats with experimental periodontitis and to explore its mechanism.Methods:30 male Sprague-Dawley rats were randomly divided into normal group(group N),periodontitis group(group P),andβ-cryptoxanthin intervention group(group E),with 10 rats of each.Experimental periodontitis model was induced by ligation of bilateral maxillary second molars cervix and injection with lipopolysaccharide(LPS).At the same time,β-cryptoxanthin(12μl/each)was injected in group E at the same site.All groups were treated every 48 hours for three times.Blood was collected and the bilateral maxilla were immediately separated on day 7,the right part of the tissues were subjected to histological analysis,serum and the left gingival tissues were detected by ELISA for IL-1β,TNF-α,and PGE2.Results:The levels of IL-1β,TNF-αand PGE2 in group E and group P were higher than those in group N,the infiltration of inflammatory cells was increased and the alveolar bone absorption was obvious.Compared with group P,the levels of IL-1β,TNF-α,and PGE2 in group E were significantly lower,the infiltration of inflammatory cells was less,and the absorption of alveolar bone was mild.Conclusion:β-cryptoxanthin can decrease the level of inflammatory factors and the inflammatory cells infiltration in tissues,thereby reducing the inflammation of periodontal tissues and alleviating alveolar bone resorption.