饥饿及恢复喂食对日本医蛭肠道菌群多样性的影响

EFFECTS OF FASTING AND REFEEDING ON HIRUDO NIPPONIA INTESTINAL MICROFLORA DIVERSITY

为了研究饥饿及恢复喂食对日本医蛭肠道微生物菌群的影响,采用高通量测序技术分析了正常组、饥饿30d、恢复喂食7d以及恢复喂食14d的日本医蛭肠道微生物菌群特征及多样性的变化。结果显示:日本医蛭核心菌群门水平上,主要集中于变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes),属水平核心菌群有气单胞菌属(Aeromonas)、Mucinivorans属、拟杆菌属(Bacteroides)、丹毒丝菌属(Erysipelothrix)等。饥饿及恢复摄食,对气单胞菌属、Mucinivorans属在内的核心菌群多样性及丰度产生了一定的影响。本研究为揭示日本医蛭肠道菌群组成以及研究日本医蛭消化道微生物功能奠定了基础。

In this study, we utilized high-throughput sequencing technology to explore thechanges of intestinal microbiota of Hirudo nipponia among fasting group, refeeding groups and control group. The results showed that (1) on the hierarchical level of the phylum, the microflora mainly include Proteobacteria, Bacteroidetes and Firmicutes;(2) on the hierarchical level of genera, the dominate microflora include Aeromonas, Mucinivorans, Bacteroides and Erysipelothrix. The results also indicated that fasting and refeeding diversity and abundance of the dominate microflora, including Aeromonas and Mucinivorans. This study lays the foundation for more detailed composition and function of digestive tract microflora in H. nipponia.