摘要
Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32min,determined by single-factor and response surface methodology(RSM)optimization.The content of polyphenol was 5.66mg/g in the crude extract.Further extraction showed that the polyphenol mainly distributed in ethyl acetate(SKEE)and water phases(SKEW).The anti-oxidation test by electron spin resonance(ESR)spectrum showed that the SKEE had the strongest scavenging activity on DPPH(1,1-diphenyl-2-picrylhydrazyl)and alkyl radicals.SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS,showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells.SKEE also signifi cantly inhibited the release of NO of LPS-induced RAW 264.7 cells.Therefore,the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-infl ammatory activities,which is beneficial to the development of high-value bio-substances.
Polypehnol is an important, potentially bioactive component of Sargassum muticum. In this study, ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38% ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32 min, determined by single-factor and response surface methodology（RSM） optimization. The content of polyphenol was 5.66 mg/g in the crude extract. Further extraction showed that the polyphenol mainly distributed in ethyl acetate（SKEE） and water phases（SKEW）.The anti-oxidation test by electron spin resonance（ESR） spectrum showed that the SKEE had the strongest scavenging activity on DPPH（1,1-diphenyl-2-picrylhydrazyl） and alkyl radicals. SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS, showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells. SKEE also significantly inhibited the release of NO of LPS-induced RAW 264.7 cells. Therefore, the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-inflammatory activities, which is beneficial to the development of high-value bio-substances.
基金
Supported by the Public Science and Technology Research Funds Projects of Ocean(No.201505022)
the Shandong Province Key Research and Development Project(No.2017YYSP003)
the Natural Science Foundation of Shandong Province(No.ZR2017MD006)
