摘要
为进一步研究水稻OsTZF3基因的生物学功能,利用日本晴基因组的OsTZF3基因序列,构建了OsTZF3的CRISP/Cas9敲除表达载体pKTZF3和过量表达载体pCXUN-TZF3;并通过农杆菌介导法导入粳稻日本晴胚性愈伤组织中,分别获得了pKTZF3和pCXUN-TZF3转化再生植株55个和42个克隆;进一步通过PCR法鉴定了35个克隆转pKTZF3植株和10个克隆转pCXUN-TZF3植株。
In order to further study the biological function of OsTZF3 gene in rice, the CRISP/Cas9 knockout expression vector pKTZF3 and overexpression vector pCXUN-TZF3 of OsTZF3 were constructed by using the sequence of OsTZF3 gene in Nipponbare. 55 transformants of pKTZF3 and 42 clones of pCXUN-TZF3 were obtained through Agrobacterium-mediated transformation. Among them, 35 clones of pKTZF3 and 10 clones of pCXUN-TZF3 were further identified by PCR detection.
作者
周淑芬
刘华清
ZHOU Shu-fen;LIU Hua-qing(Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003;Fujian Key Laboratory of Agricultural Genetic Engineering, Fuzhou, Fujian 350003)
出处
《福建农业科技》
2019年第3期1-5,共5页
Fujian Agricultural Science and Technology
基金
福建省自然科学基金项目(2015J01107)
福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1019-3)
