RUNX3基因甲基化在结直肠癌发生中作用及与预后的关系研究

The role of UNX3 gene methylation in the development of colorectal cancer and its relationship with prognosis

目的分析结直肠癌中RUNX3基因甲基化状态,探究基因甲基化与疾病预后的关系。方法选取59例结直肠癌组织、正常黏膜组织,以免疫组化法检测组织中RUNX3蛋白表达,采用甲基化特异性PCR技术(MSP)检测RUNX3甲基化状态,观察不同组织中RUNX3蛋白表达及甲基化状态,分析基因甲基化与疾病预后的关系。结果癌组织中RUNX3蛋白阳性表达为49.15%低于正常组织94.92%,同时RUNX3甲基化阳性40.68%高于正常组织0,P <0.05;淋巴结转移、RUNX3甲基化状态与患者存活有关,P <0.05,与年龄、分期、分化程度无关,P> 0.05。结论 RUNX3蛋白表达、基因甲基化状态在CRC癌组织、正常组织中存在差异,RUNX3基因甲基化状态可能为疾病发生、预后判断提供依据。

Objective To analyze the methylation status of RUNX3 gene in colorectal cancer and explore the relationship between gene methylation and disease prognosis. Methods Fifty-nine cases of colorectal cancer tissues and normal mucosa were taken.The expression of RUNX3 protein was detected by immunohistochemistry. The methylation status of RUNX3 was detected by methylation-specific PCR(MSP).The RUNX3 protein in different tissues was observed.Expression and methylation status,analysis of the relationship between gene methylation and disease prognosis. Results The positive expression of RUNX3 protein in cancer tissues was 49.15% lower than that in normal tissues,94.92%,while the positive RUNX3 methylation was 40.68% higher than that in normal tissues 0, P < 0.05.Lymph node metastasis and RUNX3 methylation status were related to patient survival,P < 0.05,regardless of age,stage,and degree of differentiation,P > 0.05. Conclusion RUNX3 protein expression and gene methylation status are different in CRC cancer tissues and normal tissues.The methylation status of RUNX3 gene may provide a basis for disease occurrence and prognosis.