转基因ABP9玉米株系的耐盐性分析

Characterization of the Salt Tolerance of Transgenic Maize Line Expressing ABP9

旨为分析转基因ABP9玉米的耐盐性,并进一步研究玉米耐盐的分子调控机制。通过对转基因ABP9玉米植株进行PCR、Southern blot和qRT-PCR分析鉴定;采用Hoagland营养液水培法,对转ABP9基因玉米及非转基因对照进行NaCl胁迫处理,分析二者在幼苗期的生理指标和基因表达差异。结果表明,转入的ABP9以单拷贝整合到基因组中,且能较高表达。与非转基因对照相比,转基因玉米幼苗NaCl胁迫下的叶绿素含量、Fv/Fm、渗透调节物质和抗氧化酶活性,以及盐胁迫后恢复过程中的叶片相对含水量均显著提高;而其丙二醛含量、相对电导率均显著降低。转录组测序和qRT-PCR分析显示,一系列盐胁迫应答基因在转基因玉米植株中上调表达。转入ABP9的增强表达提高了转基因玉米的耐盐能力。

This work is to analyze the salt tolerance of ABP9 transgenic maize,and to study the molecular regulation mechanism of salt tolerance in maize. ABP9 transgenic plants were identified by PCR,Southern blot and quantitative Reverse Transcription-PCR(qRT-PCR),and their responses at seedling stage to NaCl treatments in Hoagland nutrient solution were molecularly and physiologically characterized with non-transgenic plants as control. The results showed that the ABP9 transgene was integrated into the genome in a single copy and expressed at a higher level. Compared with the non-transgenic control,chlorophyll content,Fv/Fm,osmotic adjustment substance,and antioxidant enzyme activities in transgenic maize seedlings under NaCl stress,and the relative water content of leaves during recovery after salt stress all significantly increased,while the aldehyde content and relative conductivity significantly reduced. Several salt stress responsive genes were upregulated in transgenic plants revealed by transcriptome profiling and qRT-PCR analysis. Our results indicate that enhanced ABP9 expression level contributes to salt tolerance in transgenic maize,providing a valuable germplasm for maize salt tolerance breeding.