二氧化氯对玉米乙醇发酵糟液中脱氧雪腐镰刀菌烯醇的降解

Degradation of deoxynivalenol by ClO_2 during ethanol fermentation of corn distiller grains

脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)是造成粮食污染的主要原因之一。采用高效液相色谱-质谱联用(HPLC-MS)对DON含量进行检测分析,通过液体浸泡法,探究了ClO_2对DON纯品以及玉米乙醇发酵糟液中DON的降解效果。结果显示:DON降解率随ClO_2溶液浓度的增大以及处理时间的延长而提高,且pH值对DON降解有影响。当pH值为4. 5～5. 5时,600 mg/L ClO_2溶液对DON纯品处理12 h,降解率为28. 23%,并产生了2种降解产物;该方法同样适用于玉米乙醇发酵糟液中DON降解,600 mg/L的ClO_2溶液对玉米发酵糟液处理12 h,降解率达40. 70%,降解效果较好,对处理乙醇发酵工艺中的DON或其他真菌毒素具有一定的指导意义,为大规模生产中对DON的去除提供了新的思路。

Deoxynivalenol( DON) is one of the main causes of food contamination. High performance liquid chromatography-mass spectrometry( HPLC-MS) was used to analyze the DON content. The degradation effects of ClO2 on pure DON and DON in corn ethanol fermentation broth were investigated. The results showed that the degradation rate of DON increased with increasing concentration of ClO2 and prolonging treatment time. Besides,pH had important effects on DON degradation. At pH 4. 5-5. 5,the degradation rate of pure DON was 28. 23% by 600 mg/L ClO2 for 12 h,and two degradation products were produced. This method was also applicable to degrade DON in corn ethanol fermentation broth and resulted better results,as the degradation rate reached 40. 70%. This study has guiding significance for treating DON or other mycotoxins during ethanol fermentation process and provides a new idea for removing DON during large-scale production.