深绿木霉D16生物菌肥对丹参生长和次生代谢的影响

Effects of fungal biofertilizer from Trichoderma atroviride D16 on the growth and secondary metabolism of Salvia miltiorrhiza

目的研究深绿木霉D16固体菌肥不同剂量在盆栽和大田环境下对丹参苗生长和次生代谢的影响,为深绿木霉生物菌肥在生产上的应用提供参考。方法分别在温室和陕西商洛丹参规范化生产基地开展实验,设置不同剂量(5、15、25 g)的D16菌肥处理组3组,25 g不加菌的固体菌种培养基为对照组,测定不同剂量D16菌肥对丹参苗生物量、有效成分含量的影响。结果不同剂量的D16菌肥对丹参生长和有效成分积累均有显著影响,施用15 g的菌肥用量效果较优,收获时在盆栽和大田环境下干重分别是同期对照组的5.61倍和1.42倍,有效成分含量最高可达盆栽同期对照组的27.91倍和大田同期对照组的1.89倍。结论 15 g的深绿木霉菌肥能显著促进丹参苗的生长和有效成分积累,为内生真菌提升中药质量的生产实践做出了有益的探索。

Objective To investigate effects of different dosages of fungal biofertilizer from Trichoderma atroviride D16 on the growth and secondary metabolism of Salvia miltiorrhiza.Methods S.miltiorrhiza and fungal biofertilizer from T.atroviride D16 were served as test materials.Effects of different D16 dosages on biomass and active compounds accumulation were studied.Results Different D16 fertilizer dosages significantly influenced the growth and secondary metabolism of S.miltiorrhiza.15 g D16 exhibited the best results.The dry weights of S.miltiorrhiza. harvested from potted and field were 5.61 and 1.42 times of the weights from corresponding control groups.The maximum level of active compounds were increased 27.91 times compared with the corresponding control in the potted and 1.89 times in the field.Conclusion 15 g D16 fungal biofertilizer enhanced the growth and secondary metabolism of S.miltiorrhiza significantly. This study offered an exploratory evidence for the quality and productivity improvement of traditional Chinese medicine with endophytic fungi.