苦参碱衍生物抗关节炎作用研究

Anti-arthritis effects of matrine derivative in rats

目的研究苦参碱衍生物M19对二型胶原诱导性大鼠关节炎(CIA)的治疗作用。方法将60只大鼠随机分为6组,分别为空白组,模型组,吲哚美辛治疗组,M19低、中、高剂量(2.5、5、10 mg/kg)组。采用二型胶原诱导建立关节炎模型,分别对大鼠足趾肿胀和体重进行测定并对关节炎进行评分,HE染色观察大鼠关节软骨及滑膜组织病理变化,Micro-CT观察大鼠骨关节组织损伤情况。结果苦参碱衍生物M19各剂量组均能不同程度降低大鼠关节炎足趾肿胀程度,增加体重,抑制大鼠关节炎性细胞浸润和滑膜增生,并对骨组织具有一定的保护作用。结论苦参碱衍生物M19对二型胶原诱导的关节炎具有一定的治疗作用。

Objective To study the therapeutic effects of matrine derivative M19 on type Ⅱcollagen-induced arthritis(CIA) in rats.Methods Sixty rats were randomly divided into six groups:blank group,model group,indomethacin treatment group,M19 low,medium and high dose groups(2.5,5,10 mg/kg).The paw swelling,arthritis index(AI) and body weight were measured for CIA rats.The histopathological changes of joints and synovial tissue were observed by HE staining.The bone destruction was observed with micro-CT.Results Matrine derivative M19 reduced the paw swelling,increased body weight,inhibited inflammatory cell infiltration,ameliorated synovial tissue and bone destruction in CIA rats.Conclusion Matrine derivative M19 has the certain therapeutic effects on CIA.