TLRs途径在日本血吸虫病肝虫卵肉芽肿形成中的作用

The study on role of TLRs signal pathway in liver eggs granuloma formation of schistosomasis japonicum

本文探讨TLRs信号途径是否为日本血吸虫抗原分子的模式识别受体,参与血吸虫病肝虫卵肉芽肿的炎症病变。采用日本血吸虫抗原分子刺激小鼠腹腔巨噬细胞,ELISA及Western blot分别检测细胞因子和NF-κB的变化状况。构建日本血吸虫病肝虫卵肉芽肿的小鼠模型,小鼠肝组织中TLR2、TLR4蛋白及mRNA的变化情况分别使用FCM和Real-time PCR检测。经抗原分子刺激后巨噬细胞分泌IL-1β、TNF-α和IL-6显著增高,与PBS组比较差异显著(P<0. 01)。IκB蛋白表达水平在刺激30 min后显著降低。Balb/c小鼠感染日本血吸虫后TLR2、TLR4蛋白及其mRNA含量以及IL-1β、TNF-α、IL-6、ALT与AST含量均显著性升高,与未感染组比较变化显著(P<0. 05)。表明TLRs信号途径可能是日本血吸虫抗原分子的模式识别受体,并被其激活;而且TLRs信号途径在血吸虫病肝虫卵肉芽肿的形成中发挥重要作用。

This paper investigates whether the TLRs signaling pathway is a pattern recognition receptor for Schistosoma japonicum antigen molecules and is involved in inflammatory lesions of schistosomiasis liver granuloma. Schistosoma japonicum antigen molecules simulation of peritoneal macrophages, and the changes of cytokines and NF-κB were detected by ELISA and Western blot, respectively. To establish the model of liver eggs granuloma of Schistosomasis japonicum in Balb/c mice. The changes of TLR2, TLR4 protein and mRNA in mouse liver tissues were detected by FCM and Real-time PCR, respectively. The secretion of IL-1β, TNF-α and IL-6 by macrophages was significantly increased after stimulation by antigen molecules, and the difference was significant compared with PBS group( P <0.01). IκB protein expression levels were significantly reduced after 30 min of stimulation. The content of TLR2, TLR4 protein and mRNA and the contents of IL-1β, TNF-α, IL- 6, ALT and AST in Balb/c mice infected with Schistosoma japonicum were significantly increased, and the changes were significant compared with the uninfected group( P <0.05). It is suggested that the TLRs signaling pathway may be the pattern recognition receptor of Schistosoma japonicum antigen molecules and is activated by it, and the TLRs signaling pathway plays an important role in the formation of schistosomiasis liver granuloma.