加味四君子汤对脾虚犬小肠组织形态及EGF表达的影响

Effect of Supplementary Sijunzi Decoction on the Tissue Morphology and EGF Expression of Small Intestine in Splenic Asthenia Canines

【目的】探究加味四君子汤对脾虚犬小肠组织形态及EGF表达的影响。【方法】选择1岁龄的比格犬24只,随机分为4组,每组6只,分别为对照组、脾虚组、自然恢复组、中药恢复组。对照组犬饲喂基础饲粮;脾虚组、自然恢复组、中药恢复组犬每天以6 mL/kg番泻叶灌喂造模。第7天,将脾虚组犬解剖采样,中药恢复组在基础饲粮中添加2%加味四君子汤,自然恢复组饲喂基础饲粮。第14天,全部犬解剖采样。采集犬十二指肠、空肠中段、回肠进行HE染色,测量肠绒毛长度、隐窝深度,计算V/C值;检测小肠EGF蛋白表达量以及mRNA相对表达量。【结果】与对照组相比,脾虚组犬小肠出现弥漫性的肠道黏膜充血、水肿,并有大量黏液渗出;小肠绒毛稀疏、变短;肠绒毛长度极显著降低(P<0.01),隐窝深度极显著升高(P<0.01),十二指肠、回肠V/C值显著降低(P<0.05),空肠V/C值极显著降低(P<0.01);小肠EGF的蛋白表达量极显著降低(P<0.01),mRNA表达量显著降低(P<0.05)。用加味四君子汤治疗后,中药恢复组各项指标均恢复到接近对照组水平。【结论】加味四君子汤能够使番泻叶诱导犬脾虚证的小肠组织形态、EGF蛋白表达量以及mRNA相对表达量恢复正常,对犬脾虚证有明显的治疗效果。

[Purpose]The present study was conducted to investigate the effects of supplementary Sijunzi decoction on the tissue morphology and EGF expression of small intestine in splenic asthenia canines.[Method]Twenty-four one-year-old beagles were selected and randomly divided into four groups (6 canines in each group). The four groups were control group, splenic asthenia group, natural recovery group and Chinese medicine recovery group, respectively. The canines in control group were fed a basal diet. The canines in splenic asthenia group, natural recovery group and Chinese medicine recovery group were fed with Senna at 6 mL/kg a day. On the 7th day, the canines in splenic asthenia group were dissected for collecting samples, the canines in Chinese medicine recovery group were fed a basal diet with 2% supplementary Sijunzi decoction, and in natural recovery group were fed a basal diet. On the 14th day, all canines were dissected for collecting samples. Duodenum, jejunum and ileum of canines were collected to measure villus length, crypt depth and V/C values after HE staining and to detect EGF protein expression and EGF mRNA relative expression levels in small intestine.[Result] Compared with the canines in control group, the diffuse congestion and edema appeared in the small intestinal mucosa of canines in splenic asthenia group, and a large amount of mucous oozed out. The villi were sparser and shorter in small intestines, villus length extremely significantly decreased (P<0.01), crypt depth extremely significantly increased (P<0.01), V/C of duodenum and ileum significantly decreased (P<0.05) and V/C of jejunum extremely significantly decreased (P<0.01). EGF protein expression in small intestine significantly decreased (P<0.05) and EGF mRNA relative expression levels in small intestine extremely significantly decreased (P<0.01). After treating by supplementary Sijunzi decoction, the above indexes of the canines in Chinese medicine recovery group recovered close to the control group.[Conclusion]Supplementary Sijunzi decoction can improve the tissue morphology, EGF protein expression and EGF mRNA relative expression levels in small intestine, and has obvious effects on treating the canines with splenic asthenia syndrome.