由甲酸-贝壳溶液湿法纺制优质丝素蛋白长丝

Wet-spun High Quality Silk Fibroin Filaments in Formic Acid-shell Solution

对于丝素蛋白(SF)柔性长丝的制备,溶解是重要的。将贝壳(shells)废物溶解在甲酸中,在室温下制备离子液体,以溶解SF纤维得到柔性长丝。结果表明,SF长丝是Silk I和Silk II结构共存。当纺丝溶液的浓度为8.0wt%至14.0wt%时,丝素蛋白溶液具有良好的可纺性。当纺丝溶液的浓度为C=12%时,长丝表面平整光滑,是湿纺丝溶液最佳纺丝浓度。我们选用去离子水和不同浓度的乙醇溶液作为凝固浴,应用于SF长丝机械性能固化,发现凝固浴的选择对丝素蛋白长丝成型有影响。在不同溶剂体系之间存在差异,SF长丝结构随溶剂体系的变化而变化,不同溶剂制备的SF长丝的形态差异明显。由于溶剂体系中离子的机理不同,SF分子在各个溶剂体系中水解不同,产生不同的水解SF分子链。以水作为凝固浴,由甲酸(FA)-贝壳(Shells)溶液湿法纺制的优质SF长丝的应力和应变分别为15.37MP±1.05MP和66.26%±2.71%,高于天然蚕丝。

For the preparation of silk fibroin(SF) flexible filaments, dissolution is important. The shells waste was dissolved in formic acid and an ionic liquid was prepared at room temperature to dissolve the SF fibers to obtain flexible filaments. The results showed that the SF filaments coexisted in the structure of Silk I and Silk II. When the concentration of the spinning solution was from 8.0 wt% to 14.0 wt%, the silk fibroin solution had good spinnability. When the concentration of the spinning solution was C=12%, the surface of the filament was even and smooth, which was the optimum spinning concentration of the wet spinning solution. The deionized water and different concentrations of ethanol solution were selected as coagulation baths, which were applied to solidifying the mechanical properties of SF filaments. It was found that the choice of coagulation bath had an effect on the formation of silk fibroin filaments. There were differences between different solvent systems, and the structure of SF filaments changed with the change of solvent system. The morphologies of SF filaments prepared by different solvents were obviously different. Due to the different mechanisms of ions in the solvent systems, the SF molecules were hydrolyzed differently in each solvent system, resulting in different hydrolyzed SF molecular chains. With water as the coagulation bath, the stress and strain of high quality SF filaments wet-spun in formic acid(FA)-shell(Shells) solution were 15.37±1.05 MP and 66.26±2.71% respectively, which was higher than natural silk.