性病梅毒患者实施外周血检验的应用价值

Application of peripheral blood test in patients with venereal syphilis

目的探讨性病梅毒患者实施外周血检验的应用价值。方法随机选取2016年1月至2018年1月海宁市中心医院和浙江省血液中心共同诊治的100例性病梅毒患者作为研究对象。将这些患者作为性病梅毒组,另选取同期来海宁市中心医院和浙江省血液中心进行健康体检的健康人100例作为健康对照组。对两组人员的外周血细胞形态、血清IL-2、IL-4、IFN-γ、TNF-α水平、外周血T淋巴细胞亚群和NK细胞进行统计分析。结果性病梅毒组患者的外周血细胞正常比例74.0%(74/100)显著低于健康对照组98.0%(98/100),差异具有统计学意义(P<0.05),细胞性贫血、白细胞变化、异型淋巴细胞比例5.0%(5/100)、8.0%(8/100)、13.0%(13/100)均显著高于健康对照组0、1.0%(1/100)、1.0%(1/100),差异均具有统计学意义(均P<0.05),血清IL-2、IL-4、IFN-γ、TNF-α水平均显著高于健康对照组,差异均具有统计学意义(均P<0.05),外周血CD_(4+)、CD_(4+)/CD_(8+)、NK均显著低于健康对照组,差异均具有统计学意义(均P<0.05),CD_(8+)显著高于健康对照组,差异具有统计学意义(P<0.05),但两组人员的外周血CD_(3+)之间的差异不显著,差异无统计学意义(P>0.05)。结论性病梅毒患者实施外周血检验的应用价值高,具有较为快速便捷的操作,联合血细胞形态和生化分析更能为临床鉴定和诊断性病梅毒提供有效依据,值得在临床推广应用。

ObjectiveTo investigate the value of peripheral blood tests in patients with venereal syphilis. Methods100 patients withvenereal syphilis admitted to Haining Central Hospital and Zhejiang Blood Center from January 2016 to January 2018 were randomly selected. These patients were treated as venereal syphilis group. Another 100 healthy people in our hospital during the same period were selected as healthy control group. Peripheral blood cell morphology, serum IL-2, IL-4, IFN-γ, TNF-α levels, peripheral blood T lymphocyte subsets and NK cells in two groups were statistically analyzed. ResultsThe percentage of normal peripheral blood cells in patients with venereal syphilis (74.0%, 74/100) was significantly lower than that of the healthy controls (98.0%, 98/100), with statistically significant difference (P<0.05). The proportion of cellular anemia, leukocytosis, and atypical lymphocyte was 5.0%(1/100), 8.0%(8/100) and 13.0%(13/100) respectively in the venereal syphilis group, significantly higher than those of the control group of 0, 1.0%(1/100) and 1.0%(1/100) respectively, with statistically significant differences (P<0.05). Theserum levels of IL-2, IL-4, IFN-γ and TNF-α in venereal syphilis group were significantly higher than those in the control group, with statically significant differences (P<0.05). The levels of CD4+, CD4+/ CD8+ and NK in peripheral blood in venereal syphilis group were significantly lower than those in the control groupand the level of CD8+ was significantly higher than that of the control group, all with statistically significant differences (P<0.05), but there was no significant difference between the two groups in the level of CD3+ in peripheral blood (P>0.05). ConclusionsThe application of peripheral blood test in patients with venereal syphilis is of high value with a quick and easy operation. Combining the blood cell morphology and biochemical analysis can provide an effective basis for clinical identification and diagnosis of syphilis, which is worthy of clinical application.