不同剂量X射线全身照射对小鼠部分免疫器官的影响

Effects of X-ray Irradiation with Different Dosages on Immune Organs in Mouse

目的探讨不同剂量X射线辐照后对小鼠免疫器官的影响。方法 BALB/c小鼠192只,随机分为正常对照组和单次照射2.0 Gy、3.0 Gy、4.0 Gy、5.0 Gy、6.0 Gy组。分别于照射后24 h、48 h、96 h、192 h每组取8只小鼠颈椎脱臼处死后取胸腺和脾脏称重,计算胸腺指数和脾脏指数。并测定组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果相同取样时间,各辐照剂量组小鼠胸腺、脾脏指数及SOD含量与对照组比较均显著降低,且与辐照剂量呈正相关。MDA含量与对照组比较均显著升高,且均有明显剂量效应。相同辐照剂量,不同取样时间,各组小鼠胸腺、脾脏指数均呈现先降低后升高趋势。胸腺中MDA含量随取样时间延长呈升高趋势,脾脏中MDA含量呈先升高后降低趋势。胸腺中SOD含量随取样时间延长呈先升高后降低趋势;辐照2.0 Gy、3.0 Gy、4.0 Gy组小鼠脾脏中SOD含量随取样时间延长呈升高趋势,辐照5.0 Gy、6.0 Gy组小鼠脾脏中SOD含量随取样时间延长呈先升高后降低趋势。结论在所观察照射剂量和测定时间内,X射线对小鼠免疫器官的影响与辐照剂量相关。不同组织重量恢复时间不同,且低剂量辐照开始恢复时间先于高剂量辐照。

Objective To explore the effects of different irradiation dosages of X-rays on mouse immune organs. Methods Totally 192 mouse were randomly divided into 6 groups: the normal control group and single irradiation 2.0 Gy group, 3.0 Gy group, 4.0 Gy group, 5.0 Gy group and 6.0 Gy group.The samples were taken respectively from mouse of each group at 24 h, 48 h, 96 h, 192 h after irradiation,and the thymus glands and spleens were weighed and taken for calculating organ index and determining the content of superoxide dismutase(SOD) and malondialdehyde(MDA). Results At the same sampling time, the thymus glands and spleen index and the content of SOD in each dose group were significantly less than control group, The content of MDA was significantly higher than control group.The trends of change in thymus glands and spleen index of the mouse in each group increased and decreased first at the same irradiation dosages and different sampling time. The content of MDA in thymus glands increased with the prolongation of sampling time, and the content of MDA in spleen was a trend of reducing the rise first. The content of SOD in thymus increased first and then decreased with the prolongation of sampling time. The content of SOD in the spleen of irradiated 2.0 Gy, 3.0 Gy and 4.0 Gy mouse increased with the sampling time. And in the irradiation of 5.0 Gy, 6.0 Gy group, the content of SOD in the spleen of mouse increased first and then decreased with the prolongation of sampling time. Conclusion The effect of X-rays on immune organs in mouse was positively correlated with the dose of irradiation during the observed dose and measurement time. Different tissue weight recovery times are different, and low-dose irradiation begins to recover time. Higher dose irradiation begins to recover earlier.