摘要
油酸和亚油酸是花生中的主要不饱和脂肪酸,油酸具有降低高血脂症患者血脂水平以及预防心血管疾病的作用,且油酸/亚油酸(O/L)是决定花生油货架期的重要指标.因此,油酸与亚油酸的含量测定对于花生的品质评价具有重要意义.采用气相色谱法,以十七烷酸甲酯为内标,对脂肪酸甲酯的制备方法进行比较与优化,建立了花生中油酸与亚油酸的含量测定方法.经方法学验证,该法专属性强、准确度高、重复性好、操作简便,适用于花生中油酸与亚油酸的定量分析.
Oleic acid and linoleic acid are the main unsaturated fatty acid in peanuts.Oleic acid has a distinct effect to reduce the blood lipid of the hyperlipidemia and to prevent cardiovascular diseases.In addition,the rate of oleic acid and linoleic acid (O/L) is a key index of the shelf life of the peanut oil.Therefore,quantitative analysis of oleic acid and linoleic acid plays an important role in the quality evaluation of peanuts.In this study,a method is established by gas chromatography,using methyl heptadecanoate as the internal standard.The preparation method of fatty acid methyl ester is also been compared and optimized.According to the result of method verification,this method is specific,accurate,reproducible,simple and rapid,and it is suitable for the quantitative analysis of oleic acid and linoleic acid.
作者
赵星
张嘉楠
杨华
王瑾
宋亚辉
陈四龙
李玉荣
ZHAO Xing;ZHANG Jia-nan;YANG Hua;WANG Jin;SONG Ya-hui;CHEN Si-long;LI Yu-rong(Hebei Academy of Agriculture and Forestry Sciences,Institute of Cereal and Oil Crops,Laboratory of Crop Genetics and Breeding of Hebei Province,Shijiazhuang,Hebei 050035,China;School of Chemical Engineering,Shijiazhuang University,Shijiazhuang,Hebei 050035,China;National Millet Improvement Center of China,Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang,Hebei 050035,China)
出处
《石家庄学院学报》
2019年第3期13-17,共5页
Journal of Shijiazhuang University
基金
国家自然科学基金(31301354,31201239)
河北省应用基础研究计划重点基础研究项目(14966317D)
河北省自然科学基金(C2015106043)
石家庄学院博士科研启动基金(BS14002)
关键词
花生
油酸
亚油酸
气相色谱
内标法
peanuts
oleic acid
linoleic acid
gas chromatography
internal standard method
